Human thrombospondin 2 inhibits proliferation of microvascular endothelial cells

被引：1
|
作者：
Tomii, Y
Kamochi, J
Yamazaki, H
Sawa, N
Tokunaga, T
Ohnishi, Y
Kijima, H
Ueyama, Y
Tamaoki, N
Nakamura, M [1 ]
机构：
[1] Tokai Univ, Sch Med, Dept Pathol, Isehara, Kanagawa 2591193, Japan
[2] Tokai Univ, Sch Med, Dept Internal Med, Isehara, Kanagawa 2591193, Japan
[3] Cent Inst Expt Anim, Kawasaki, Kanagawa 2160001, Japan
[4] Chugai Pharmaceut Co Ltd, Fuji Gotemba Res Labs, Shizuoka 4128513, Japan
来源：
INTERNATIONAL JOURNAL OF ONCOLOGY | 2002年 / 20卷 / 02期
关键词：
human thrombospondin 2; angiogenesis;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
This study was performed to characterize human thrombospondin 2 (TSP2). TSP2 has recently attracted attention as an endogenous negative regulator of angiogenesis in tumorigenesis. We cloned and transfected human TSP2 cDNA into the human colon cancer cell line SW-480. Stable transfectants (TSP2-1, TSP2-6) overexpressing TSP2 were established. Growth characteristics of TSP2-transfectants were investigated in vitro and in vivo. TSP2-transfectants showed similar growth properties to vector-transfectants and wild-type SW-480 cells. The overexpression of transfected human TSP2 cDNA did not affect proliferation of SW-480 cells. When the conditioned media of TSP2-transfectants were added to cultures of bovine pulmonary microvascular endothelial cells (BPMEC), the BPMEC proliferation was significantly inhibited. These results suggested that human TSP2 is a potential inhibitor of angiogenesis.
引用
收藏
页码：339 / 342
页数：4
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