Isolation and phenotypic characterization of cancer stem-like side population cells in colon cancer

被引:12
|
作者
Feng, Long [1 ]
Wu, Jian-Bing [1 ]
Yi, Feng-Ming [1 ]
机构
[1] Nanchang Univ, Dept Oncol, Affiliated Hosp 2, Nanchang 330006, Jiangxi, Peoples R China
关键词
cancer stem cells; chemotherapy; side population cells; tumor recurrence; apoptosis; TUMOR-CELLS; INTERLEUKIN-4; IDENTIFICATION; EXPRESSION; RESISTANCE; AUTOCRINE; CAPACITY; SURVIVAL; GROWTH; DEATH;
D O I
10.3892/mmr.2015.3801
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Previous studies in cancer biology suggest that chemotherapeutic drug resistance and tumor relapse are driven by cells within a tumor termed 'cancer stem cells'. In the present study, a Hoechst 33342 dye exclusion technique was used to identify cancer stem-like side population (SP) cells in colon carcinoma, which accounted for 3.4% of the total cell population. Following treatment with verapamil, the population of SP cells was reduced to 0.6%. In addition, the sorted SP cells exhibited marked multidrug resistance and enhanced cell survival rates compared with non-SP cells. The SP cells were able to generate more tumor spheres and were CD133 positive. Subsequent biochemical analysis revealed that the levels of the adenosine triphosphate-binding cassette sub-family G member 2 transporter protein, B-cell lymphoma anti-apoptotic factor and autocrine production of interleukin-4 were significantly enhanced in the colon cancer SP cells, which contributed to drug resistance, protection of the cells from apoptosis and tumor recurrence. Therefore, the findings suggested that treatment failure and colon tumorigenesis is dictated by a small population of SP cells, which indicate a potential target in future therapies.
引用
收藏
页码:3531 / 3536
页数:6
相关论文
共 50 条
  • [1] Isolation and characterization of cancer stem-like side population cells in human oral cancer cells
    Yanamoto, Souichi
    Kawasaki, Goro
    Yamada, Shin-ichi
    Yoshitomi, Izumi
    Kawano, Toshihiro
    Yonezawa, Hisanobu
    Rokutanda, Satoshi
    Naruse, Tomofumi
    Umeda, Masahiro
    [J]. ORAL ONCOLOGY, 2011, 47 (09) : 855 - 860
  • [2] Isolation and Identification of Cancer Stem-like Cells from Side Population of Human Prostate Cancer Cells
    陈雅童
    赵佳晖
    罗勇
    王永兴
    韦能宝
    姜永光
    [J]. Journal of Huazhong University of Science and Technology(Medical Sciences), 2012, 32 (05) : 697 - 703
  • [3] Isolation and identification of cancer stem-like cells from side population of human prostate cancer cells
    Chen, Yatong
    Zhao, Jiahui
    Luo, Yong
    Wang, Yongxing
    Wei, Nengbao
    Jiang, Yongguang
    [J]. JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES, 2012, 32 (05) : 697 - 703
  • [4] Isolation and identification of cancer stem-like cells from side population of human prostate cancer cells
    Yatong Chen
    Jiahui Zhao
    Yong Luo
    Yongxing Wang
    Nengbao Wei
    Yongguang Jiang
    [J]. Journal of Huazhong University of Science and Technology [Medical Sciences], 2012, 32 : 697 - 703
  • [5] The side population of cancer stem-like cells in human oral cancer
    Chien, Chih-Yen
    Chuang, Hui-Ching
    Chen, Chang-Han
    [J]. ORAL ONCOLOGY, 2012, 48 (10) : 913 - 914
  • [6] Side population cells define a stem-like compartment in ovarian cancer
    Boesch, M.
    Reimer, D.
    Zeimet, A. G.
    Sopper, S.
    Wolf, D.
    [J]. ONKOLOGIE, 2012, 35 : 203 - 204
  • [7] Characterization of side population in thyroid cancer cell lines: Cancer stem-like cells are enriched partly but not exclusively
    Mitsutake, Norisato
    Iwao, Atsuhiko
    Nagai, Kazuhiro
    Namba, Hiroyuki
    Ohtsuru, Akira
    Saenko, Vladimir
    Yamashita, Shunichi
    [J]. ENDOCRINOLOGY, 2007, 148 (04) : 1797 - 1803
  • [8] Isolation of colorectal cancer stem-like cells
    Eunice Dotse
    Yuhong Bian
    [J]. Cytotechnology, 2016, 68 : 609 - 619
  • [9] Isolation of colorectal cancer stem-like cells
    Dotse, Eunice
    Bian, Yuhong
    [J]. CYTOTECHNOLOGY, 2016, 68 (04) : 609 - 619
  • [10] Characterization of cancer stem-like cells in the side population cells of human gastric cancer cell line MKN-45
    Hai-hong ZHANG
    Ai-zhen CA
    Xue-ming WEI
    Li DING
    Feng-zhi LI
    Ai-ming ZHENG
    Da-jiang DAI
    Rong-rong HUANG
    Hou-jun CAO
    Hai-yang ZHOU
    Jian-mei WANG
    Xue-jing WANG
    Wei SHI
    Heng ZHU
    Xiao-ying YUAN
    Lin CHEN
    [J]. Journal of Zhejiang University-Science B(Biomedicine & Biotechnology), 2013, (03) : 216 - 223