Isolation of tetracycline-resistant clinical Helicobacter pylori without mutations in 16S rRNA gene in Bangladesh

被引：8

作者：

Khan, Rasel ^{[1
,2
]}

Nahar, Shamsun ^{[2
]}

Mukhopadhyay, Asish K. ^{[4
]}

Berg, Douglas E. ^{[5
,6
,7
]}

Ahmad, Mian Mashhud ^{[3
]}

Okamoto, Keinosuke ^{[1
]}

Nair, G. Balakrish ^{[2
]}

Rahman, Motiur ^{[2
]}

机构：

[1] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Okayama 7008530, Japan

[2] B Ctr Hlth & Populat Res, ICDDR B, Div Sci Lab, Dhaka, Bangladesh

[3] Dhaka Med Coll Hosp, Dhaka, Bangladesh

[4] Natl Inst Cholera & Enter Dis, Kolkata, India

[5] Washington Univ, Sch Med, Dept Mol Microbiol, St Louis, MO 63110 USA

[6] Washington Univ, Sch Med, Dept Genet, St Louis, MO 63110 USA

[7] Washington Univ, Sch Med, Dept Med, St Louis, MO 63110 USA

来源：

MICROBIOLOGY AND IMMUNOLOGY | 2008年 / 52卷 / 10期

关键词：

Helicobacter pylori; resistance; tetracycline; 16S rRNA gene mutation;

D O I：

10.1111/j.1348-0421.2008.00062.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The occurrence of 16S rRNA gene mutations associated with resistance to tetracycline in H. pylori isolated in Bangladesh was investigated. Tetracycline susceptibility was determined by the agar dilution method. The 16S rRNA genes of these isolates were sequenced and analyzed. A tetracycline accumulation assay was performed. DNA sequence and transformation tests of nine tetracycline-resistant (MIC = 2 mu g/ml) Bangladeshi H. pylori clinical isolates showed that in no case was the resistance due to mutations in the 16S rRNA gene, the only known cause of tetracycline resistance in this pathogen. Tetracycline accumulation assays implicated altered uptake or efflux.

引用

页码：508 / 511

页数：4

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