Proteomic identification of Listeria monocytogenes surface-associated proteins

被引：10
|
作者：
Zhang, Cathy X. Y. [1 ,2 ]
Creskey, Marybeth C. [3 ]
Cyr, Terry D. [3 ]
Brooks, Brian [1 ]
Huang, Hongsheng [1 ]
Pagotto, Franco [2 ,4 ]
Lin, Min [1 ,2 ]
机构：
[1] Canadian Food Inspect Agcy, Ottawa Lab Fallowfield, Ottawa, ON K2H 8P9, Canada
[2] Univ Ottawa, Dept Biochem Microbiol & Immunol, Ottawa, ON, Canada
[3] Hlth Canada, Ctr Vaccine Evaluat, Ottawa, ON K1A 0L2, Canada
[4] Hlth Canada, Bur Microbial Hazards, Listeriosis Reference Serv, Ottawa, ON K1A 0L2, Canada
来源：
PROTEOMICS | 2013年 / 13卷 / 20期
关键词：
Listeria monocytogenes; Microbiology; Protein digest; Protein identification; TANDEM MASS-SPECTROMETRY; SEROTYPE; 4B; STATISTICAL-MODEL; CHROMATOGRAPHY; PEPTIDOGLYCAN; INACTIVATION; ANTIGENS; DATABASE; CANCER; CORE;
D O I：
10.1002/pmic.201200449
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This study aimed to identify proteins exposed on the surface of Listeria monocytogenes cells for diagnostic reagent development. Brief trypsin treatment of L. monocytogenes cells followed by peptide separation and identification by nano-LC and online-MS/MS was performed. In parallel, as a negative control, proteins secreted into the digest buffer as well as proteins from cell lysis were identified. One hundred and seventy-four proteins were identified in at least two of three trials in either the negative control or during cell digest. Nineteen surface, 21 extracellularly secreted, 132 cytoplasmic, and two phage proteins were identified. Immunofluorescence microscopy of L. monocytogenes cells revealed the surface localization of two potential candidates for L. monocytogenes isolation and detection: lipoprotein LMOf2365_0546 and PBPD1 (LMOf2365_2742). In this report, we present the first data set of surface-exposed L. monocytogenes proteins currently available. The data have been deposited to the ProteomeXchange Consortium with identifier PXD000035.
引用
收藏
页码：3040 / 3045
页数：6
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