Detection of Rickettsia in Rhipicephalus sanguineus Ticks and Ctenocephalides felis Fleas from Southeastern Tunisia by Reverse Line Blot Assay

被引:24
|
作者
Khrouf, Fatma [1 ]
M'Ghirbi, Youmna [1 ]
Znazen, Abir [2 ]
Ben Jemaa, Mounir [3 ]
Hammami, Adnene [2 ]
Bouattour, Ali [1 ]
机构
[1] Univ Tunis El Manar, Inst Pasteur Tunis, Tunis, Tunisia
[2] Univ Sfax, Hop Habib Bourguiba, Sfax, Tunisia
[3] Univ Sfax, Hop Hedi Chaker, Sfax, Tunisia
关键词
FEVER GROUP RICKETTSIAE; 1ST MOLECULAR-DETECTION; MEDITERRANEAN SPOTTED-FEVER; PHYLOGENETIC ANALYSIS; IDENTIFICATION; CONORII; BORNE; MASSILIAE; DISEASES; ALGERIA;
D O I
10.1128/JCM.01925-13
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Ticks (n = 663) and fleas (n = 470) collected from domestic animals from southeastern Tunisia were screened for Rickettsia infection using reverse line blot assay. Evidence of spotted fever group Rickettsia was obtained. We detected Rickettsia felis in fleas, Rickettsia massiliae Bar 29 and the Rickettsia conorii Israeli spotted fever strain in ticks, and Rickettsia conorii subsp. conorii and Rickettsia spp. in both arthropods. The sensitivity of the adopted technique allowed the identification of a new association between fleas and R. conorii subsp. conorii species. The presence of these vector-borne Rickettsia infections should be considered when diagnosing this disease in humans in Tunisia.
引用
收藏
页码:268 / 274
页数:7
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