High-Resolution Differential Ion Mobility Spectrometry of a Protein

被引：27
|
作者：
Shvartsburg, Alexandre A. [1 ]
Smith, Richard D. [1 ]
机构：
[1] Pacific NW Natl Lab, Div Biol Sci, Richland, WA 99352 USA
来源：
ANALYTICAL CHEMISTRY | 2013年 / 85卷 / 01期
关键词：
GAS-PHASE SEPARATIONS; UBIQUITIN CONFORMERS; MASS SPECTROMETRY; CROSS-SECTIONS; FIELD; ANALYZERS; CONFORMATIONS; TEMPERATURE; DISTORTION;
D O I：
10.1021/ac3029129
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Use of elevated electric fields and helium-rich gases has recently enabled differential ion mobility spectrometry (IMS) with a resolving power up to R 300. Here we applied that technique to a protein (ubiquitin), achieving R up to 80 and separating previously unresolved conformers. While still limited by conformational multiplicity, this resolution is some 4 times greater than that previously reported using either conventional (drift-tube or traveling-wave) or differential IMS. The capability for fine resolution of protein conformers may open new avenues for proteoform separations in top-down and intact-protein proteomics.
引用
收藏
页码：10 / 13
页数：4
相关论文
共 50 条
  • [1] Exploration of high-resolution differential ion mobility spectrometry for large proteins
    Shvartsburg, Alexandre
    [J]. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2015, 250
  • [2] High-Resolution Ion Cyclotron Mobility Spectrometry
    Merenbloom, Samuel I.
    Glaskin, Rebecca S.
    Henson, Zachary B.
    Clemmer, David E.
    [J]. ANALYTICAL CHEMISTRY, 2009, 81 (04) : 1482 - 1487
  • [3] Broad Separation of Isomeric Lipids by High-Resolution Differential Ion Mobility Spectrometry with Tandem Mass Spectrometry
    Bowman, Andrew P.
    Abzalimov, Rinat R.
    Shvartsburg, Alexandre A.
    [J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2017, 28 (08) : 1552 - 1561
  • [4] Electrospray ionization high-resolution ion mobility spectrometry - Mass spectrometry
    Wu, C
    Siems, WF
    Asbury, GR
    Hill, HH
    [J]. ANALYTICAL CHEMISTRY, 1998, 70 (23) : 4929 - 4938
  • [5] Separation of Peptide Isomers with Variant Modified Sites by High-Resolution Differential Ion Mobility Spectrometry
    Shvartsburg, Alexandre A.
    Creese, Andrew J.
    Smith, Richard D.
    Cooper, Helen J.
    [J]. ANALYTICAL CHEMISTRY, 2010, 82 (19) : 8327 - 8334
  • [6] Rapid separation of isomeric perfluoroalkyl substances by high-resolution differential ion mobility mass spectrometry
    Ahmed, Ezaz
    Kabir, K. M. Mohibul
    Wang, Huixin
    Xiao, Dan
    Fletcher, John
    Donald, William A.
    [J]. ANALYTICA CHIMICA ACTA, 2019, 1058 : 127 - 135
  • [7] High-Resolution Differential Ion Mobility Separations/Orbitrap Mass Spectrometry without Buffer Gas Limitations
    Baird, Matthew A.
    Shliaha, Pavel V.
    Anderson, Gordon A.
    Moskovets, Eugene
    Laiko, Victor
    Makarov, Alexander A.
    Jensen, Ole N.
    Shvartsburg, Alexandre A.
    [J]. ANALYTICAL CHEMISTRY, 2019, 91 (10) : 6918 - 6925
  • [8] High-resolution differential ion mobility separations with tandem mass spectrometry using Orbitrap and its applications
    Shvartsburg, Alexandre A.
    Moore, Ronald J.
    Smith, Richard D.
    [J]. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2013, 246
  • [9] High-Resolution Ion Mobility Spectrometry for Rapid Cannabis Potency Testing
    Hadener, Marianne
    Kamrath, Michael Z.
    Weinmann, Wolfgang
    Groessl, Michael
    [J]. ANALYTICAL CHEMISTRY, 2018, 90 (15) : 8764 - 8768
  • [10] High-Resolution Ion Mobility Spectrometry-Mass Spectrometry on Poly(methyl methacrylate)
    Song, Junkan
    Grun, Christian H.
    Heeren, Ron M. A.
    Janssen, Hans-Gerd
    van den Brink, Oscar F.
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2010, 49 (52) : 10168 - 10171
12345