Occurrence of Theileria equi in horses raised in the Jaboticabal microregion, Sao Paulo State, Brazil

被引:22
|
作者
Baldani, Cristiane Divan [1 ]
Higa Nakaghi, Andrea Cristina [2 ]
Machado, Rosangela Zacarias [2 ]
机构
[1] Univ Fed Rural Rio de Janeiro UFRRJ, Lab Patol Clin, Dept Med & Cirurgia Vet, Inst Vet, BR-23890000 Seropedica, RJ, Brazil
[2] Univ Estadual Paulista UNESP, Fac Ciencias Agr & Vet, Dept Patol Vet, Sao Paulo, Brazil
来源
基金
巴西圣保罗研究基金会;
关键词
Theileria equi; horse; diagnosis; IFAT; nested PCR;
D O I
10.1590/S1984-29612010000400007
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Blood and serum samples from 170 horses raised in the Jaboticabal microregion, Sao Paulo State, Brazil, were collected and tested by microscopic examination of blood smears, indirect fluorescent antibody test (IFAT) and nested polymerase chain reaction (nPCR) for Theileria equi infections. The association among the test results was verified by the McNemar test. During the examination of thin blood smears, parasites were detected in six (3.52%) horses. Anti-T. equi antibodies were detected in 100% sera samples, with titers ranging between 1:80 and 1:5120. The nPCR based on the T equi merozoite antigen gene (EMA-1) allowed the visualization of specie-specific amplified product in 108 (63.53%) horses. All six samples judged positive microscopically were also positive for nPCR. Statistical analysis indicated general disagreement (p < 0.0001) between IFAT and nPCR; IFAT and blood smear; and nPCR and blood smear on the detection of parasite carriers. The results of the present study indicate that T equi is widely spread among horses in the Jaboticabal microregion, Northeast region of Sao Paulo State, Brazil.
引用
收藏
页码:228 / 232
页数:5
相关论文
共 50 条
  • [1] Seroprevalence of and risk factors for Toxoplasma gondii in sheep raised in the Jaboticabal microregion, Sao Paulo State, Brazil
    Zanetti Lopes, Welber Daniel
    dos Santos, Thais Rabelo
    da Silva, Ricardo dos Santos
    Rossanese, Walter Matheus
    de Souza, Fernando Augusto
    de Faria Rodrigues, Joana D'Ark
    de Mendonca, Rafael Paranhos
    Soares, Vando Edesio
    da Costa, Alvimar Jose
    [J]. RESEARCH IN VETERINARY SCIENCE, 2010, 88 (01) : 104 - 106
  • [2] The Palm Collection of UNESP/FCAV - Jaboticabal, Sao Paulo State, Brazil
    Pivetta, K. F. L.
    Bueno, B. F.
    Batista, G. S.
    Mazzini, R. B.
    Gimenes, R.
    Romani, G. de N.
    Pimenta, R. S.
    [J]. I INTERNATIONAL SYMPOSIUM ON GENETIC RESOURCES OF BAMBOOS AND PALMS AND III INTERNATIONAL SYMPOSIUM ON ORNAMENTAL PALMS, 2013, 1003 : 221 - 226
  • [3] Risk factors to tick infestations and their occurrence on horses in the state of Sao Paulo, Brazil
    Labruna, MB
    Kerber, CE
    Ferreira, F
    Faccini, JLH
    De Waal, DT
    Gennari, SM
    [J]. VETERINARY PARASITOLOGY, 2001, 97 (01) : 1 - 14
  • [4] Influence of the relief and erosion in the space variability of an oxissol in Jaboticabal, state of Sao Paulo, Brazil
    Souza, CK
    Junior, JM
    Filho, MVM
    Pereira, GT
    [J]. REVISTA BRASILEIRA DE CIENCIA DO SOLO, 2003, 27 (06): : 1067 - 1074
  • [5] Production of recombinant EMA-1 protein and its application for the diagnosis of Theileria equi using an enzyme immunoassay in horses from Sao Paulo State, Brazil
    Baldani, Cristiane Divan
    Hilario, Eduardo
    Higa Nakaghi, Andrea Cristina
    Bertolini, Maria Celia
    Machado, Rosangela Zacarias
    [J]. REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA, 2011, 20 (01): : 54 - 60
  • [6] Helminth fauna of sheep from the micro region of Jaboticabal, Sao Paulo State, Brazil
    Maciel, Willian Giquelin
    Felippelli, Gustavo
    Zanetti Lopes, Welber Daniel
    Pires Teixeira, Weslen Fabricio
    Cruz, Breno Cayeiro
    dos Santos, Thais Rabelo
    Buzzulini, Carolina
    Favero, Flavia
    Gomes, Lucas Costa
    de Oliveira, Gilson Pereira
    da Costa, Alvimar Jose
    Shigaki de Matos, Lucas Vinicius
    [J]. CIENCIA RURAL, 2014, 44 (03): : 492 - 497
  • [7] Occurrence of Plant-Parasitic Nematodes in Ornamental and Flowering Plants at UNESP/FCAV, Campus of Jaboticabal, Sao Paulo State, Brazil
    Gimenes, R.
    Batista, G. S.
    Pivetta, K. F. L.
    Santos, J. M.
    Soares, P. L. M.
    Martins, T. A.
    [J]. II INTERNATIONAL CONFERENCE ON LANDSCAPE AND URBAN HORTICULTURE, 2010, 881 : 607 - 610
  • [8] Agrometeorological models for groundnut crop yield forecasting in the Jaboticabal, Sao Paulo State region, Brazil
    Moreto, Victor Brunini
    Rolim, Glauco de Souza
    [J]. ACTA SCIENTIARUM-AGRONOMY, 2015, 37 (04): : 403 - 410
  • [9] B-mode and M-mode Echocardiography of endurance horses raised in Sao Paulo state, Brazil
    Michima, LED
    Latorre, SM
    de Andrade, AFC
    Fernandes, WR
    [J]. JOURNAL OF EQUINE VETERINARY SCIENCE, 2004, 24 (10) : 451 - 457
  • [10] Bayesian analysis for estimating the return period of maximum precipitation at Jaboticabal Sao Paulo state, Brazil
    Beijo, Luiz Alberto
    Ferrua Vivanco, Mario Javier
    Muniz, Joel Augusto
    [J]. CIENCIA E AGROTECNOLOGIA, 2009, 33 (01): : 261 - 270