Molecular and biochemical characterization of tomato farnesyl-protein transferase

被引:23
|
作者
Schmitt, D [1 ]
Callan, K [1 ]
Gruissem, W [1 ]
机构
[1] UNIV CALIF BERKELEY,DEPT PLANT BIOL,BERKELEY,CA 94720
关键词
D O I
10.1104/pp.112.2.767
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The prenylation of membrane-associated proteins involved in the regulation of eukaryotic cell growth and signal transduction is critically important for their subcellular localization and biological activity. In contrast to mammalian cells and yeast, however, the function of protein prenylation in plants is not well understood and only a few prenylated proteins have been identified. We partially purified and characterized farnesyl-protein transferase from tomato (Lycopersicon esculentum, LeFTase) to analyze its biochemical and molecular properties. Using Ras- and G gamma-specific peptide substrates and competition assays we showed that tomato protein extracts have both farnesyl-protein transferase and geranylgeranyl-protein transferase 1 activities. Compared with the heterologous synthetic peptide substrates, the plant-specific CaaX sequence of the ANJ1 protein is a less efficient substrate for LeFTase in vitro. LeFTase activity profiles and LeFTase beta-subunit protein (LeFTB) levels differ significantly in various tissues and are regulated during fruit development. Partially purified LeFTase requires Zn2+ and Mg2+ for enzymatic activity and has an apparent molecular mass of 100 kD. Immunoprecipitation experiments using anti-alpha LeFTB antibodies confirmed that LeFTB is a component of LeFTase but not of tomato geranylgeranyl-protein transferase 1. Based on their conserved biochemical activities, we expect that prenyltransferases are likely integrated with the sterol biosynthesis pathway in the control of plant cell growth.
引用
收藏
页码:767 / 777
页数:11
相关论文
共 50 条
  • [1] INHIBITORS OF FARNESYL-PROTEIN TRANSFERASE
    GRAHAM, SL
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1995, 210 : 4 - ORGN
  • [2] THE STEREOCHEMICAL COURSE OF FARNESYL-PROTEIN TRANSFERASE
    GIBBS, RA
    MU, YO
    OMER, CA
    POMPLIANO, DL
    GIBBS, JB
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1994, 208 : 77 - MEDI
  • [3] Diarylether inhibitors of farnesyl-protein transferase
    Dinsmore, CJ
    Williams, TM
    Hamilton, K
    ONeill, TJ
    Rands, E
    Koblan, KS
    Kohl, NE
    Gibbs, JB
    Graham, SL
    Hartman, GD
    Oliff, AI
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 1997, 7 (10) : 1345 - 1348
  • [4] PURIFICATION AND CHARACTERIZATION OF FARNESYL-PROTEIN TRANSFERASE FROM BOVINE TESTIS
    RYU, KY
    BAIK, YJ
    YANG, CH
    JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1995, 28 (03): : 197 - 203
  • [5] PSEUDOPEPTIDE INHIBITORS OF RAS FARNESYL-PROTEIN TRANSFERASE
    GRAHAM, SL
    DESOLMS, SJ
    GIULIANI, EA
    KOHL, NE
    MOSSER, SD
    OLIFF, AI
    POMPLIANO, DL
    RANDS, E
    BRESLIN, MJ
    DEANA, AA
    GARSKY, VM
    SCHOLZ, TH
    GIBBS, JB
    SMITH, RL
    JOURNAL OF MEDICINAL CHEMISTRY, 1994, 37 (06) : 725 - 732
  • [6] PSEUDOPEPTIDE INHIBITORS OF RAS FARNESYL-PROTEIN TRANSFERASE
    DESOLMS, SJ
    GIULIANI, EA
    GRAHAM, SL
    ANTHONY, NJ
    GOMEZ, RP
    HOLTZ, WJ
    KOHL, NE
    LEE, TJ
    MOSSER, SD
    OLIFF, AI
    POMPLIANO, DL
    RANDS, E
    SCHOLZ, TH
    GIBBS, JB
    SMITH, RL
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1994, 207 : 277 - MEDI
  • [7] Diaryl ether inhibitors of farnesyl-protein transferase
    MacTough, SC
    DeSolms, SJ
    Shaw, AW
    Abrams, MT
    Ciccarone, TR
    Davide, JP
    Hamilton, KA
    Hutchinson, JH
    Koblan, KS
    Kohl, NE
    Lobell, RB
    Robinson, RG
    Graham, SL
    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2001, 11 (10) : 1257 - 1260
  • [8] ISOPRENYLATION OF THE RAS ONCOGENE PROTEIN BY A FARNESYL-PROTEIN TRANSFERASE
    GIBBS, JB
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1992, 203 : 9 - BTEC
  • [9] POLYISOPRENYLATION OF RAS INVITRO BY A FARNESYL-PROTEIN TRANSFERASE
    SCHABER, MD
    OHARA, MB
    GARSKY, VM
    MOSSER, SD
    BERGSTROM, JD
    MOORES, SL
    MARSHALL, MS
    FRIEDMAN, PA
    DIXON, RAF
    GIBBS, JB
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1990, 265 (25) : 14701 - 14704
  • [10] INHIBITION OF FARNESYL-PROTEIN TRANSFERASE BY GLIOTOXIN AND ACETYLGLIOTOXIN
    VANDERPYL, D
    INOKOSHI, J
    SHIOMI, K
    YANG, H
    TAKESHIMA, H
    OMURA, S
    JOURNAL OF ANTIBIOTICS, 1992, 45 (11): : 1802 - 1805