A T7 exonuclease-assisted cyclic enzymatic amplification method coupled with rolling circle amplification: a dual-amplification strategy for sensitive and selective microRNA detection

被引：65

作者：

Cui, Liang ^{[1
]}

Zhu, Zhi ^{[1
]}

Lin, Ninghang ^{[1
]}

Zhang, Huimin ^{[1
]}

Guan, Zhichao ^{[1
]}

Yang, Chaoyong James ^{[1
]}

机构：

[1] Xiamen Univ, Coll Chem & Chem Engn, Dept Biol Chem,MOE Key Lab Spectrochem Anal & Ins, State Key Lab Phys Chem Solid Surfaces,Key Lab Ch, Xiamen 361005, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2014年 / 50卷 / 13期

基金：

美国国家科学基金会;

关键词：

ULTRASENSITIVE DETECTION; MOLECULAR BEACONS; EXPRESSION; PROBES; SYSTEM; GENE;

D O I：

10.1039/c3cc48707e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A T7 exonuclease-assisted cyclic enzymatic amplification method (CEAM) was combined with rolling circle amplification (RCA) to develop a RCA-CEAM dual amplification method for ultrasensitive detection of microRNA with excellent selectivity.

引用

页码：1576 / 1578

页数：3

共 50 条

[1] Dual-Signal Amplification Strategy for Sensitive MicroRNA Detection Based on Rolling Circle Amplification and Enzymatic Repairing Amplification
Xiao, Fubing
Liu, Jie
Guo, Qinghui
Du, Zhibo
Li, Hong
Sun, Chunlong
Du, Wenfang
[J]. ACS OMEGA, 2020, 5 (50): : 32738 - 32743
[2] T7 exonuclease-assisted and target-triggered cascade dual recycling signal amplification strategy for the sensitive and specific detection of adenosine
Wang, Gang
Wang, Lei
Li, Xia
Xu, Xiaowen
Jiang, Wei
[J]. TALANTA, 2019, 197 : 234 - 238
[3] A sensitive fluorescence strategy for telomerase detection in cancer cells based on T7 exonuclease-assisted target recycling amplification
Wang, Hai-bo
Wu, Shuang
Chu, Xia
Yu, Ru-Qin
[J]. CHEMICAL COMMUNICATIONS, 2012, 48 (47) : 5916 - 5918
[4] A sensitive strategy for the fluorescence detection of DNA methyltransferase activity based on the graphene oxide platform and T7 exonuclease-assisted cyclic signal amplification
Ma, Yefei
Chen, Lini
Zhang, Liangliang
Liao, Suqi
Zhao, Jingjin
[J]. ANALYST, 2015, 140 (12) : 4076 - 4082
[5] Multiplex detection of microRNAs by combining molecular beacon probes with T7 exonuclease-assisted cyclic amplification reaction
Liu, Yacui
Zhang, Jiangyan
Tian, Jingxiao
Fan, Xiaofei
Geng, Hao
Cheng, Yongqiang
[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2017, 409 (01) : 107 - 114
[6] Multiplex detection of microRNAs by combining molecular beacon probes with T7 exonuclease-assisted cyclic amplification reaction
Yacui Liu
Jiangyan Zhang
Jingxiao Tian
Xiaofei Fan
Hao Geng
Yongqiang Cheng
[J]. Analytical and Bioanalytical Chemistry, 2017, 409 : 107 - 114
[7] Exonuclease III-Assisted Target Recycling Amplification Coupled with Liposome-Assisted Amplification: One-Step and Dual-Amplification Strategy for Highly Sensitive Fluorescence Detection of DNA
Zhou, Fulin
Li, Baoxin
[J]. ANALYTICAL CHEMISTRY, 2015, 87 (14) : 7156 - 7162
[8] Dual-probe fluorescent biosensor based on T7 exonuclease-assisted target recycling amplification for simultaneous sensitive detection of microRNA-21 and microRNA-155
Zheng, Yanjie
Chen, Jinyuan
Li, You
Xu, Yichun
Chen, Li
Chen, Wei
Liu, Ailin
Lin, Xinhua
Weng, Shaohuang
[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2021, 413 (06) : 1605 - 1614
[9] Dual-probe fluorescent biosensor based on T7 exonuclease-assisted target recycling amplification for simultaneous sensitive detection of microRNA-21 and microRNA-155
Yanjie Zheng
Jinyuan Chen
You Li
Yichun Xu
Li Chen
Wei Chen
Ailin Liu
Xinhua Lin
Shaohuang Weng
[J]. Analytical and Bioanalytical Chemistry, 2021, 413 : 1605 - 1614
[10] Ratiometric fluorescence sensor based on carbon dots as internal reference signal and T7 exonuclease-assisted signal amplification strategy for microRNA-21 detection
Wang, Zhenzhen
Xue, Zhiqiang
Hao, Xiaoli
Miao, Chenfang
Zhang, Jianzhong
Zheng, Yanjie
Zheng, Zongfu
Lin, Xinhua
Weng, Shaohuang
[J]. ANALYTICA CHIMICA ACTA, 2020, 1103 : 212 - 219

← 1 2 3 4 5 →