Comparative study of deoxynivalenol, 3-acetyldeoxynivalenol, and 15-acetyldeoxynivalenol on intestinal transport and IL-8 secretion in the human cell line Caco-2

被引：53
|
作者：
Kadota, Tomoyuki [1 ,2 ]
Furusawa, Hiroko [3 ]
Hirano, Satoshi [1 ]
Tajima, Osamu [1 ]
Kamata, Yoichi [4 ]
Sugita-Konishi, Yoshiko [5 ]
机构：
[1] Kirin Co Ltd, Div Res & Dev, Cent Labs Key Technol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan
[2] Gifu Univ, United Grad Sch Vet Sci, Gifu 5011193, Japan
[3] Natl Inst Hlth Sci, Div Microbiol, Setagaya Ku, Tokyo 1588501, Japan
[4] Iwate Univ, Fac Agr, Dept Vet Med, Morioka, Iwate 0208550, Japan
[5] Azabu Univ, Grad Sch Life & Environm Sci, Dept Food & Life Sci, Lab Food Hyg,Chuo Ku, Sagamihara, Kanagawa 2525201, Japan
来源：
TOXICOLOGY IN VITRO | 2013年 / 27卷 / 06期
关键词：
Acetyldeoxynivalenol; Trichothecene; Caco-2; cells; Transepithelial transport; IL-8; TRANSEPITHELIAL TRANSPORT; GENE-EXPRESSION; INDUCTION; DERIVATIVES; METABOLISM; MYCOTOXINS; MACROPHAGE; MONOLAYERS; VOMITOXIN; NIVALENOL;
D O I：
10.1016/j.tiv.2013.06.003
中图分类号：
R99 [毒物学（毒理学）];
学科分类号：
100405 ;
摘要：
The effects of the trichothecene mycotoxin deoxynivalenol (DON) and its acetylated derivatives, 3-acetyldeoxynivalenol (3ADON) and 15-acetyldeoxynivalenol (15ADON) on human intestinal cell Caco-2 were investigated by the studies of transepithelial transport, gene expression, and cytokine secretion. Permeability across a Caco-2 cell monolayer was evaluated by transport study. Transport rates were ranked as DON, 3ADON < 15ADON in apical-basolateral direction. 15ADON showed the highest permeability, induced the highest decrease in transepithelial electrical resistance (TEER), and prompted significant Lucifer Yellow permeability. These results showed that 15ADON affect paracellular barrier function extremely. In addition, gene expressions induced by toxins were screened by DNA microarray for investigating cellular effect on Caco-2 cell. The most remarkable gene induced by DON and 15ADON was inflammatory chemokine IL-8 and thus mRNA expression and secretion of IL-8 were analyzed by PCR and ELISA. Both DON and acetylated DONs could induce mRNA expression and production of IL-8. In particular, ELISA assay showed that the ability to produce IL-8 was ranked as 3ADON < DON < 15ADON. Our results indicated that 15ADON caused the highest permeability and highest IL-8 secretion among DON, 3ADON, and 15ADON in human intestinal cell. (c) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1888 / 1895
页数：8
相关论文
共 50 条
  • [1] Comparison of Emetic Potencies of the 8-Ketotrichothecenes Deoxynivalenol, 15-Acetyldeoxynivalenol, 3-Acetyldeoxynivalenol, Fusarenon X, and Nivalenol
    Wu, Wenda
    Bates, Melissa A.
    Bursian, Steven J.
    Link, Jane E.
    Flannery, Brenna M.
    Sugita-Konishi, Yoshiko
    Watanabe, Maiko
    Zhang, Haibin
    Pestka, James J.
    [J]. TOXICOLOGICAL SCIENCES, 2013, 131 (01) : 279 - 291
  • [2] Toxicity and metabolic effect of combined deoxynivalenol, 3-acetyldeoxy-nivalenol, and 15-acetyldeoxynivalenol on a Caco-2 cell model
    Ye, Yongli
    Wei, Kaimin
    Ji, Jian
    Ning, Xiao
    Wang, Jia-Sheng
    Sun, Xiulan
    [J]. FOOD BIOSCIENCE, 2023, 53
  • [3] DIRECT ENZYME-LINKED IMMUNOSORBENT ASSAYS FOR THE DETECTION OF THE 8-KETOTRICHOTHECENE MYCOTOXINS DEOXYNIVALENOL, 3-ACETYLDEOXYNIVALENOL, AND 15-ACETYLDEOXYNIVALENOL IN BUFFER SOLUTIONS
    USLEBER, E
    MARTLBAUER, E
    DIETRICH, R
    TERPLAN, G
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1991, 39 (11) : 2091 - 2095
  • [4] Role of Cholecystokinin in Anorexia Induction Following Oral Exposure to the 8-Ketotrichothecenes Deoxynivalenol, 15-Acetyldeoxynivalenol, 3-Acetyldeoxynivalenol, Fusarenon X, and Nivalenol
    Wu, Wenda
    Zhou, Hui-Ren
    He, Kaiyu
    Pan, Xiao
    Sugita-Konishi, Yoshiko
    Watanabe, Maiko
    Zhang, Haibin
    Pestka, James J.
    [J]. TOXICOLOGICAL SCIENCES, 2014, 138 (02) : 278 - 289
  • [5] Influence of deoxynivalenol on NF-κB activation and IL-8 secretion in human intestinal Caco-2 cells
    De Walle, Jacqueline Van
    Romier, Beatrice
    Larondelle, Yvan
    Schneider, Yves-Jacques
    [J]. TOXICOLOGY LETTERS, 2008, 177 (03) : 205 - 214
  • [6] Zinc transport and metallothionein secretion in the intestinal human cell line Caco-2
    Moltedo, O
    Verde, C
    Capasso, A
    Parisi, E
    Remondelli, P
    Bonatti, S
    Alvarez-Hernandez, X
    Glass, J
    Alvino, CG
    Leone, A
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (41) : 31819 - 31825
  • [7] Preliminary study of thalidomide transport by the human intestinal cell line Caco-2
    Zhou, SF
    Li, Y
    Kestell, P
    Paxton, JW
    [J]. DRUG METABOLISM REVIEWS, 2002, 34 : 148 - 148
  • [8] Cryptosporidium parvum infection stimulates the secretion of TGF-β, IL-8 and RANTES by Caco-2 cell line
    C. Maillot
    G. Gargala
    A. Delaunay
    P. Ducrotte
    P. Brasseur
    J. J. Ballet
    L. Favennec
    [J]. Parasitology Research, 2000, 86 : 947 - 949
  • [9] Cryptosporidium parvum infection stimulates the secretion of TGF-β, IL-8 and RANTES by Caco-2 cell line
    Maillot, C
    Gargala, G
    Delaunay, A
    Ducrotte, P
    Brasseur, P
    Ballet, JJ
    Favennec, L
    [J]. PARASITOLOGY RESEARCH, 2000, 86 (12) : 947 - 949
  • [10] Regulation of IL-6 secretion by the Caco-2 human intestinal epithelial cell line.
    McGee, DW
    Vitkus, SJD
    Hulbert, SA
    [J]. JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1997, 99 (01) : 151 - 151
12345