Activity-guided Isolation of a Novel Protein from Croton tiglium with Antifungal and Antibacterial Activities

被引：12
|
作者：
Shahid, Muhammad [2 ]
Tayyab, Muhammad [2 ]
Naz, Farah [2 ]
Jamil, Amer [2 ]
Ashraf, Muhammad [3 ]
Gilani, Anwarul Hassan [1 ]
机构：
[1] Aga Khan Univ, Coll Med, Dept Biol & Biomed Sci, Nat Prod Res Div, Karachi, Pakistan
[2] Univ Agr Faisalabad, Dept Chem & Biochem, Mol Biochem Lab, Faisalabad 38040, Pakistan
[3] Univ Agr Faisalabad, Dept Bot, Faisalabad 38040, Pakistan
来源：
PHYTOTHERAPY RESEARCH | 2008年 / 22卷 / 12期
关键词：
Croton tiglium; bioactive peptide/protein; antibacterial; antifungal;
D O I：
10.1002/ptr.2544
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
This study describes the activity-guided isolation and purification of a novel antimicrobial protein from the seed of Croton tiglium Linn. Purification was carried out by (NH4)(2)SO4 precipitation, gel filtration and DEAE-cellulose ion-exchange chromatography. Antifungal and antibacterial activities were determined after each purification step. SDS-polyacrylamide gel electrophoresis revealed that the purified protein was a monomer with molecular mass of 50 kDa. This is a first report on purification of a protein from Croton tiglium, which possesses a strong and broad spectrum antimicrobial activity. Copyright (C) 2008 John Wiley & Sons, Ltd.
引用
收藏
页码：1646 / 1649
页数：4
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