Construction and characterization of a recombinant reticuloendotheliosis virus expressing enhanced green fluorescent protein

被引：2
|
作者：
Deng, Xiaoyun [1 ,2 ,4 ]
Hu, Feng [2 ]
Qi, Xiaole [2 ]
Gao, Li [2 ]
Li, Kai [2 ]
Gao, Honglei [2 ]
Gao, Yulong [2 ]
Wang, Yongqiang [2 ]
Shen, Nan [2 ]
Hua, Yuping [1 ]
Wang, Xiaomei [2 ,3 ]
机构：
[1] Northeast Forestry Univ, Coll Wildlife Resources, Harbin 150040, Peoples R China
[2] Chinese Acad Agr Sci, Harbin Vet Res Inst, Div Avian Infect Dis, State Key Lab Vet Biotechnol, Harbin 150001, Peoples R China
[3] Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China
[4] Heilongjiang Polytech, Harbin 150111, Peoples R China
来源：
ARCHIVES OF VIROLOGY | 2015年 / 160卷 / 09期
关键词：
AVIAN-LEUKOSIS VIRUS; ENDANGERED ATTWATERS; INFECTIOUS CLONE; FOWLPOX-VIRUS; CHICKENS; VISUALIZATION; DISEASE; CHINA;
D O I：
10.1007/s00705-015-2502-3
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Reticuloendotheliosis virus (REV) causes an immunosuppressive and oncogenic disease in chickens and other birds. In this study, based on an infectious clone of REV, named HLJR0901, a recombinant virus containing the enhanced green fluorescence protein (EGFP) gene was constructed by inserting the EGFP expression cassette downstream of the 3' terminus of the viral env gene. An EGFP-tagged REV that stably expresses EGFP was rescued. This visible recombinant REV could contribute to the further understanding of the molecular mechanism involved in the replication and pathogenicity of REV.
引用
收藏
页码：2231 / 2235
页数：5
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