Antimicrobial flavonoids from Tridax procumbens

被引:10
|
作者
Jindal, Alka [1 ]
Kumar, Padma [1 ]
机构
[1] Univ Rajasthan, Dept Bot, Lab Plant Tissue Culture & Secondary Metabolites, Jaipur 302004, Rajasthan, India
关键词
T; procumbens; callus; flavonoid; antimicrobial potential; EXTRACTS; BIOACTIVITY;
D O I
10.1080/14786419.2011.617746
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Callus culture of Tridax procumbens has been established on Murashige and Skoog's medium supplemented with NAA and BAP from nodal segments. Free and bound flavonoids were extracted from 2, 4, 6 and 8 weeks old calli by a well-established method. These free flavonoids were then screened against Staphylococcus aureus (bacteria) and Candida albicans (yeast) for their antimicrobial potential. Minimum inhibitory concentration, minimum bactericidal/ fungicidal concentrations and total activity were also evaluated. Apigenin, quercetin and kaempferol were identified from free flavonoids of 4 weeks old callus (most active) through, thin layer chromatography, (TLC) preparative TLC, MP and IR spectral studies.
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页码:2072 / 2077
页数:6
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