Purification and characterization of β-glucosidase from Aspergillus niger strain 322

被引：13
|
作者：
Peshin, A [1 ]
Mathur, JMS [1 ]
机构：
[1] Indian Agr Res Inst, Dept Biochem, New Delhi 110012, India
来源：
LETTERS IN APPLIED MICROBIOLOGY | 1999年 / 28卷 / 05期
关键词：
D O I：
10.1046/j.1365-2672.1999.00533.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An extracellular beta-glucosidase enzyme was purified from the fungus. Aspergillus niger strain 322. The molecular mass of the enzyme was estimated to be 64 kDa by SDS gel electrophoresis. Optimal pH and temperature for beta-glucosidase were 5.5 and 50 degrees C, respectively. Purified enzyme was stable up to 50 degrees C and pH between 2.0 and 5.5. The K-m was 0.1 mmol l(-1) for cellobiose. Enzyme activity was inhibited by several divalent metal ions.
引用
收藏
页码：401 / 404
页数：4
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