Dorsal Horn of Mouse Lumbar Spinal Cord Imaged with CLARITY

被引：0
|
作者：
Sengul, Gulgun [1 ]
Liang, Huazheng [2 ,3 ]
Furlong, Teri M. [2 ,4 ]
Paxinos, George [2 ,4 ]
机构：
[1] Ege Univ, Sch Med, Dept Anat, TR-35100 Izmir, Turkey
[2] Neurosci Res Australia, 139 Barker St, Sydney, NSW 2031, Australia
[3] Tongji Univ, Sch Med, Dept Neurol,Shanghai Fourth Peoples Hosp, Translat Res Inst Brain & Brain Like Intelligence, Shanghai 200081, Peoples R China
[4] Univ New South Wales, Sch Med Sci, Kensington, NSW 2052, Australia
来源：
BIOMED RESEARCH INTERNATIONAL | 2020年 / 2020卷
基金：
澳大利亚研究理事会; 英国医学研究理事会;
关键词：
GENE-RELATED PEPTIDE; NITRIC-OXIDE SYNTHASE; CYTOARCHITECTONIC ORGANIZATION; IMMUNOCYTOCHEMICAL EVIDENCE; CHOLINE-ACETYLTRANSFERASE; RAT; NEURONS; LOCALIZATION; EXPRESSION; BRAIN;
D O I：
10.1155/2020/3689380
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The organization of the mouse spinal dorsal horn has been delineated in 2D for the six Rexed laminae in our publication Atlas of the Spinal Cord: Mouse, Rat, Rhesus, Marmoset, and Human. In the present study, the tissue clearing technique CLARITY was used to observe the cyto- and chemoarchitecture of the mouse spinal cord in 3D, using a variety of immunohistochemical markers. We confirm prior observations regarding the location of glycine and serotonin immunoreactivities. Novel observations include the demonstration of numerous calcitonin gene-related peptide (CGRP) perikarya, as well as CGRP fibers and terminals in all laminae of the dorsal horn. We also observed sparse choline acetyltransferase (ChAT) immunoreactivity in small perikarya and fibers and terminals in all dorsal horn laminae, while gamma aminobutyric acid (GABA) and glutamate decarboxylase-67 (GAD67) immunoreactivities were found only in small perikarya and fibers. Finally, numerous serotonergic fibers were observed in all laminae of the dorsal horn. In conclusion, CLARITY confirmed the 2D immunohistochemical properties of the spinal cord. Furthermore, we observed novel anatomical characteristics of the spinal cord and demonstrated that CLARITY can be used on spinal cord tissue to examine many proteins of interest.
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页数：8
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