Could mesenchymal stem cell therapy help in the treatment of muscle damage caused by Bothrops alternatus venom?

被引：0
|
作者：
Telles, Thalita da Costa [1 ]
Machado Botelho, Ana Flavia [1 ]
Soares de Miranda, Ana Luisa [1 ]
Soto-Blanco, Benito [1 ]
Serakides, Rogeria [1 ]
Ocarino, Natalia de Melo [1 ]
Melo, Marilia Martins [1 ]
机构：
[1] Univ Fed Minas Gerais, Dept Clin & Cirurgia Vet, Ave Antonio Carlos 6626, BR-31270901 Belo Horizonte, MG, Brazil
来源：
CIENCIA RURAL | 2018年 / 48卷 / 03期
关键词：
Viperidae envenomation; stem cells; rats; myonecrosis; muscular repair; INJECTION; REGENERATION; MICE;
D O I：
10.1590/0103-8478cr20170760
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
The aim of this study was to evaluate the use of mesenchymal stem cells (MSC) in the treatment of myonecrosis induced by Bothrops alternatus venom in rats. Seventy-five male adult Wistar rats were divided into three experimental groups. G1 and G2 were injected in the gastrocnemius muscle with 120 mu g of B. alternatus venom, while G3 received 200 mu L of PBS only. Three days after the venom injection, 12 rats from G1 were treated with 5.0 x 10(6) MSC in PBS, whereas G2 and G3 rats received PBS. Every three days, blood and muscle samples of five animals from each group were taken for serum biochemical and pathological analyses. Histological examinations showed more intense muscle lesions following MSC treatment, characterized by disorganization and loss of muscle fibers, with focal necrosis and inflammatory infiltration by mononuclear cells. In conclusion, the use of MSC for the treatment of local damage caused by inoculation of B. alternatus venom impaired muscle regeneration and interfered in the healing process.
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页数：4
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