In vivo protection studies of bis-quaternary 2-(hydroxyimino)-N-(pyridin-3-yl) acetamide derivatives against sarin poisoning in mice

被引:2
|
作者
Swami, Devyani [1 ]
Karade, Hitendra N. [2 ]
Acharya, Jyotiranjan [2 ]
Kumar, Pravin [1 ]
机构
[1] Def Res & Dev Estab, Div Pharmacol & Toxicol, Jhansi Rd, Gwalior 474002, India
[2] Def Res & Dev Estab, Proc Technol Dev Div, Gwalior, India
关键词
Sarin; GB; oximes; HNK oximes; AChE; nerve agents; ACETYLCHOLINESTERASE KNOCKOUT MOUSE; NERVE AGENT; GUINEA-PIGS; ORGANOPHOSPHORUS COMPOUNDS; HIGH-AFFINITY; REACTIVATION; RAT; CHOLINESTERASE; OXIMES; BRAIN;
D O I
10.1177/0960327116637109
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
In vivo antidotal efficacy of new bis-quaternary 2-(hydroxyimino)-N-(pyridin-3yl) acetamide derivatives (HNK series), to counter multiples of lethal doses of nerve agent sarin (GB) and reactivation of acetylcholinesterase (AChE), was evaluated in Swiss albino mice. [Protection index PI; median lethal dose (LD50) of sarin with treatment/LD50 of sarin] was estimated, using 0.05, 0.10, and 0.20 LD50 as treatment doses of all the oximes with atropine against sarin poisoning. Dose-dependent time course study was conducted at 0.2, 0.4 and 0.8 LD50 dose of sarin for estimating maximum AChE inhibition. At optimized time (15 min), in vivo enzyme half inhibition concentration (IC50) was calculated. AChE reactivation efficacy of HNK series and pralidoxime (2-PAM) were determined by plotting shift of log IC50 doses. HNK-102 with atropine showed three fold higher PI compared to 2-PAM. In vivo IC50 of sarin for brain and serum AChE was found to be 0.87 LD50 (139.2 mg/kg) and 0.48 LD50 (77.23 mg/kg), respectively. Treatment with HNK-102 and HNK-111 (equal to their 0.20LD(50)) significantly reactivated sarin-intoxicated AChE (p < 0.05) at 2x IC50 dose of sarin, compared to 2-PAM. The study revealed that HNK-102 oxime was three times more potent as antidote, for acute sarin poisoning compared to 2-PAM in vivo.
引用
收藏
页码:23 / 32
页数:10
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