Analysis of 8-hydroxydeoxyguanosine in human urine by solid-phase extraction and derivatization gas chromatography

被引：0
|
作者：
Mei, SR [1 ]
Xu, GW
Wu, CY
机构：
[1] Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromotog Res & Anal Ctr, Dalian 116011, Peoples R China
[2] Wuhan Univ, Coll Chem, Wuhan 430072, Peoples R China
来源：
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2001年 / 29卷 / 12期
关键词：
8-hydroxydeoxyguanosine; gas chromatography; solid-phase extractions; derivatization; gas chromatography/mass spectrometry;
D O I：
暂无
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A method for analysing 8-hydroxydeoxyguanosine (8OHdG) in human urine by solid-phase extraction and derivatization gas chromatography is described. The experimental conditions, especially the optimization of derivatization, were systematically studied. The 80HdG was identified by gas chromatography/mass spectrometry. The detection limit of this method is 4.56 pg/s, and the linear range is 0.2 similar to 100 mug. For the analysis of 80HdG in human urine, the RSD is less than 8.0% (n = 5).
引用
收藏
页码：1394 / 1397
页数：4
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