The new Rapid-i carrier is an effective system for human embryo vitrification at both the blastocyst and cleavage stage

被引:48
|
作者
Desai, Nina N. [1 ]
Goldberg, Jeffrey M. [1 ]
Austin, Cynthia [1 ]
Falcone, Tommaso [1 ]
机构
[1] Cleveland Clin, Fertil Ctr, Womens Hlth Inst, Dept OB,GYN, Beachwood, OH USA
来源
关键词
PREGNANCY OUTCOMES; CRYOPRESERVATION; SURVIVAL; OOCYTES; CONTAMINATION; COLLAPSE; MOUSE; CYCLES;
D O I
10.1186/1477-7827-11-41
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background: The Rapid-i is a new FDA cleared closed carrier for embryo vitrification. The cooling rate of - 1220 degrees C/min is far lower than that reported with open vitrification systems such as the cryoloop (-15,000 C/min). Little published data is currently available on this device. This study presents our initial clinical data, as well as live birth outcomes, with the Rapid-i. The efficacy of this device for the cryopreservation of cleavage, as well as blastocyst stage human embryos is also analyzed. We further compare outcomes to those achieved with the cryoloop, an "open" vitrification system routinely used in our laboratory. Methods: Human embryos were vitrified at either the 8-10 cell stage or else the blastocyst stage. The vitrification protocol was: 7.5% DMSO/7.5% ethylene glycol (EG) (2-3 min) followed by incubation in 15% DMSO/15% EG (45 sec) before loading on the vitrification carrier. Cryoprotectant was removed during warming by sequential washes in 0.25 M and 0.125 M sucrose in culture medium. Clinical outcome data for frozen cycles between January 2011 and August 2012 were stratified according to carrier and cell stage. The student t-test and chi square test were used to compare results. P value of < 0.05 was considered significant. Results: A total of 486 vitrified-warmed embryos were assessed and 92% of them were transferred. The clinical pregnancy rate (CPR) and implantation rate (IR) with Rapid-i vitrified blastocysts were 59% and 49%, versus 47% and 37%, respectively for cleavage stage embryos. This was not statistically different from results with the cryoloop vitrified blastocysts (CPR 46%, IR 38%) nor the cleavage stage vitrified embryos (CPR 49%, IR 35%). To date, there have been 31 deliveries of 34 healthy infants from Rapid-i vitrified embryos, with another 12 pregnancies still on-going. Conclusions: The Rapid-i offers an excellent alternative to existing open vitrification devices for embryo cryopreservation at the 8-10 cell stage as well as the blastocyst stage. Use of this type of "closed" sealed system that prevents direct contact between the embryos and liquid nitrogen reduces the potential risk of sample cross-contamination or infection. These preliminary data and live birth outcomes have paved the way toward transitioning to a closed vitrification system in our own IVF program.
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页数:9
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  • [1] The new Rapid-i carrier is an effective system for human embryo vitrification at both the blastocyst and cleavage stage
    Nina N Desai
    Jeffrey M Goldberg
    Cynthia Austin
    Tommaso Falcone
    [J]. Reproductive Biology and Endocrinology, 11
  • [2] The new Rapid i carrier is an effective closed system for human embryo vitrification at both the blastocyst and cleavage stage
    Desai, N.
    Goldberg, J.
    Austin, C.
    Falcone, T.
    [J]. HUMAN REPRODUCTION, 2012, 27
  • [3] The new Rapid i carrier is effective for human embryo vitrification at both the cleavage and blastocyst stage: preliminary clinical pregnancy and live birth outcomes
    Desai, N.
    Austin, C.
    Goldberg, J.
    Falcone, T.
    [J]. HUMAN REPRODUCTION, 2013, 28 : 113 - 114
  • [4] THE NEW RAPID I CLOSED VITRIFICATION SYSTEM IS TECHNICALLY EASY TO USE AND GIVES EXCELLENT OUTCOMES WITH BOTH BLASTOCYST AND CLEAVAGE STAGE EMBRYOS
    Desai, N.
    Goldberg, J.
    Austin, C.
    [J]. FERTILITY AND STERILITY, 2012, 98 (03) : S129 - S130
  • [5] VITRIFICATION OF HUMAN AND MOUSE EMBRYOS USING THE RAPID-I™.
    Balaban, B.
    Isiklar, A.
    Urman, B.
    Gardner, D. K.
    Larman, M. G.
    [J]. FERTILITY AND STERILITY, 2010, 94 (04) : S115 - S115
  • [6] Effect of mouse ovarian tissue cryopreservation by vitrification with Rapid-i closed system
    Naoki Okamoto
    Mariko Nakajima
    Yodo Sugishita
    Nao Suzuki
    [J]. Journal of Assisted Reproduction and Genetics, 2018, 35 : 607 - 613
  • [7] WHAT IS THE OPTIMAL STAGE FOR EMBRYO VITRIFICATION-A COMPARISON OF EMBRYO SURVIVAL AND CLINICAL OUTCOMES WITH DAY 3 CLEAVAGE VERSUS BLASTOCYST STAGE VITRIFICATION.
    Desai, N.
    Falcone, T.
    Goldberg, J.
    Austin, C.
    Goldfarb, J.
    [J]. FERTILITY AND STERILITY, 2010, 94 (04) : S110 - S110
  • [8] Effect of mouse ovarian tissue cryopreservation by vitrification with Rapid-i closed system
    Okamoto, Naoki
    Nakajima, Mariko
    Sugishita, Yodo
    Suzuki, Nao
    [J]. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, 2018, 35 (04) : 607 - 613
  • [9] APPLICATION FOR CRYOPRESERVATION OF A SMALL NUMBER OF HUMAN SPERMATOZOA IN A CLOSED SYSTEM BY USING RAPID-I™ VIA VITRIFICATION
    Egashira, A.
    Murakami, M.
    Tanaka, K.
    Otsubo, H.
    Matsuguma, T.
    Kuramoto, T.
    [J]. FERTILITY AND STERILITY, 2011, 96 (03) : S215 - S216
  • [10] Simple effective vitrification of a small number of human spermatozoa using Rapid-i carriers: a follow-up study
    Murakami, M.
    Mizumoto, S.
    Tanaka, K.
    Otsubo, H.
    Egashira, A.
    Kuramoto, T.
    [J]. HUMAN REPRODUCTION, 2016, 31 : 141 - 141