Fluviimonas pallidilutea gen. nov., sp nov., a new member of the family Cytophagaceae isolated from a freshwater river

A bacterial strain designated TQQ6(T) was isolated from a freshwater river in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain TQQ6(T) were strictly aerobic, Gram-staining-negative, poly-beta-hydroxybutyrate-containing, non-motile, non-spore-forming, long rods surrounded by a thick capsule and forming pale orange colonies. Growth occurred at 20- 40 degrees C (optimum, 25 degrees C), at pH 7.0-9.0 (optimum, pH 8.0) and with 0-0.5% NaCl (optimum, 0 %). The predominant fatty acids were iso-C-15:0, summed feature 3 (comprising C-16:1 omega 6c and/ or C-16:1 omega 7c), iso-C-17:0 3-OH, C-16:1 omega 5c and C-16:0.The major isoprenoid quinone was MK-7 and the DNA G+C content was 42.2 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, two uncharacterized aminophospholipids and three uncharacterized phospholipids. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain TQQ6(T) represents a distinct phyletic line that reflects a novel generic status within the family Cytophagaceae with relatively low sequence similarities (less than 90%) to members of other genera with validly published names. On the basis of the genotypic and phenotypic data, strain TQQ6(T) represents a new genus and novel species of the family Cytophagaceae, for which the name Fluviimonas pallidilutea gen. nov., sp. nov. is proposed. The type strain is TQQ6(T) (=BCRC 80447(T)=LMG 27056(T)=KCTC 32035(T)).