Pathotype and Genetic Diversity amongst Indian Isolates of Xanthomonas oryzae pv. oryzae

被引:55
|
作者
Mishra, Deo [1 ]
Vishnupriya, Manne Ramachander [2 ]
Anil, Madhusoodana Girija [2 ]
Konda, Kotilingam [1 ]
Raj, Yog [1 ]
Sonti, Ramesh V. [2 ]
机构
[1] Bayer BioSci, Hyderabad, Andhra Pradesh, India
[2] CSIR, Ctr Cellular & Mol Biol, Hyderabad, Andhra Pradesh, India
来源
PLOS ONE | 2013年 / 8卷 / 11期
关键词
BACTERIAL-BLIGHT RESISTANCE; MARKER-ASSISTED SELECTION; DISEASE-SUSCEPTIBILITY GENE; RICE VARIETY; POPULATION; INDUCTION; PATHOGEN; TREES; RFLP; XA13;
D O I
10.1371/journal.pone.0081996
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A number of rice resistance genes, called Xa genes, have been identified that confer resistance against various strains of Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial blight. An understanding of pathotype diversity within the target pathogen population is required for identifying the Xa genes that are to be deployed for development of resistant rice cultivars. Among 1024 isolates of Xoo collected from 20 different states of India, 11 major pathotypes were distinguished based on their reaction towards ten Xa genes (Xa1, Xa3, Xa4, xa5, Xa7, xa8, Xa10, Xa11, xa13, Xa21). Isolates belonging to pathotype III showing incompatible interaction towards xa8, xa13 and Xa21 and compatible interaction towards the rest of Xa genes formed the most frequent (41%) and widely distributed pathotype. The vast majority of the assayed Xoo isolates were incompatible with one or more Xa genes. Exceptionally, the isolates of pathotype XI were virulent on all Xa genes, but have restricted distribution. Considering the individual R-genes, Xa21 appeared as the most broadly effective, conferring resistance against 88 % of the isolates, followed in decreasing order by xa13 (84 %), xa8 (64 %), xa5 (30 %), Xa7 (17 %) and Xa4 (14 %). Fifty isolates representing all the eleven pathotypes were analyzed by southern hybridization to determine their genetic relatedness using the IS1112 repeat element of Xoo. Isolates belonging to pathotype XI were the most divergent. The results suggest that one RFLP haplotype that is widely distributed all over India and is represented in strains from five different pathotypes might be an ancestral hapfotype. A rice line with xa5, xa13 and Xa21 resistance genes is resistant to all strains, including those belonging to pathotype XI. This three gene combination appears to be the most suitable Xa gene combination to be deployed in Indian rice cultivars.
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页数:11
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