Histamine-Producing Bacteria and Histamine Induction in Retail Sardine and Mackerel from Fish Markets in Egypt

This study examined the occurrence of histamine-producing bacteria (HPB) and histamine induction in retail sardine and mackerel in Egypt; and whether the fish vendors play a role in the transmission of HPB. Fish were collected from the fish markets, additionally; hand swab samples were taken from the fish vendors. All samples were cultured on modified Niven's medium (MNM); the positive colonies were subcultured on Violet Red Bile Glucose (VRBG) agar, followed by biochemical identification and histidine decarboxylase (hdc)-gene-PCR of the VRBG-positive isolates. The hdc-gene-positive fish and human isolates were subjected to partial hdc-gene-sequencing and phylogenetic analysis. Production of histamine in the fish muscles was measured by high-performance liquid chromatography. A higher percentage of sardine showed the presence of MNM-positive bacteria (84%) than mackerel (53%). Enterobacteriaceae was the dominant family; the most frequent species were Enterobacter cloacae, Raoultella planticola, Citrobacter freundii, and Enterobacter aerogenes. Higher proportion of the R. planticola isolates were hdc positive as compared with the other species. Only 32% sardine and 17% mackerel of the MNM-positive isolates carried the hdc gene. Fish muscles that contain hdc-positive bacteria exhibit higher levels of histamine (median 86; IQR 80-1112 mg/kg) than those with hdc-negative bacteria (48; 75-223 mg/kg). The level of histamine was significantly higher in sardine (109; 104-1094 mg/kg) than in mackerel (40; 49-106 mg/kg). The 20 fish vendor samples were MNM positive, 2 of them were hdc-gene positive. The close genetic relatedness between the human and fish strains isolated from the same markets suggests a possible bidirectional transmission of the HPB. This warns for the presence of HPB carrying hdc gene in retail sardine and mackerel, which is associated with a relatively high level of histamine. Regular inspection of the fish markets is required, including accurate determination of HPB by using a combination of the MNM culture, hdc-gene PCR, and measurement of histamine level.