Lifetime of M intermediate in the D96N mutant of bacteriorhodopsin determined by a photoelectrochemical method

被引：5
|
作者：
Koyama, K [1 ]
Miyasaka, T
Needleman, R
Lanyi, JK
机构：
[1] Fuji Photo Film Co Ltd, Ashigara Res Labs, Kanagawa 2500193, Japan
[2] Wayne State Univ, Dept Chem & Mol Biol, Detroit, MI USA
[3] Univ Calif Irvine, Dept Physiol & Biophys, Irvine, CA 92717 USA
来源：
CHEMISTRY LETTERS | 1999年 / 08期
关键词：
D O I：
10.1246/cl.1999.769
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The recombinant D96N mutant of bacteriorhodopsin (bR) was immobilized on SnO2 electrode that contacts an aqueous electrolyte. By laser pulse excitation, photocurrent behavior of D96N was analyzed to evaluate the lifetime or the M intermediate involved in proton transfer reactions. The half-life of the M intermediate in the photocycle was about 5 s, which is 10(3)-fold longer than that of wild-type and shows the crucial role of D96 in the uptake of protons.
引用
收藏
页码：769 / 770
页数：2
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