A quantitative inverse relationship between connexin32 expression and cell proliferation in a rat hepatoma cell line

被引:13
|
作者
Edwards, Gareth Owain [1 ]
Jondhale, Shrikant [1 ]
Chen, Tao [1 ]
Chipman, J. Kevin [1 ]
机构
[1] Univ Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, England
关键词
Gap junction; Connexin32; Proliferation; Non-genotoxic carcinogen; RNA interference;
D O I
10.1016/j.tox.2008.08.010
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Gap junctions comprised of connexin proteins are involved in direct intercellular communication and the regulation of cell behaviour and homeostasis. Reduced connexin expression and loss of gap junction function is a characteristic of many cancer cells and of the effect of many non-genotoxic carcinogens that induce cell proliferation. Moreover, when certain cancer cell lines are transfected with specific connexin genes, cells can regain control over proliferation. We have employed RNA interference and dexamethasone to modulate connexin32 expression in MH1C1 cells to a range of concentrations. This allowed the determination of the quantitative relationship between connexin32 Protein expression and cell proliferation. The magnitude of cell proliferation, measured by bromodeoxyuridine incorporation, was inversely proportional to the level of connexin32 expression. Q-PCR indicated a lack of change of expression of a range of cell cycle-related genes at 24 h. The inverse relationship between Cx32 expression and proliferation was continuous, and a threshold level of reduction of connexin32 was not observable for an influence on proliferation. (c) 2008 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:46 / 52
页数:7
相关论文
共 50 条
  • [1] Connexin32 regulates hepatoma cell metastasis and proliferation via the p53 and Akt pathways
    Zhao, Bixing
    Zhao, Wenxiu
    Wang, Yu
    Xu, Yaping
    Xu, Jianfeng
    Tang, Kai
    Zhang, Sheng
    Yin, Zhenyu
    Wu, Qiao
    Wang, Xiaomin
    ONCOTARGET, 2015, 6 (12) : 10116 - 10133
  • [2] Connexin32 as a tumor suppressor gene in a metastatic renal cell carcinoma cell line
    Eriko Fujimoto
    Hiromi Sato
    Sumiko Shirai
    Yoji Nagashima
    Keiko Fukumoto
    Hiromi Hagiwara
    Etsuko Negishi
    Koichi Ueno
    Yasufumi Omori
    Hiroshi Yamasaki
    Kiyokazu Hagiwara
    Tomohiro Yano
    Oncogene, 2005, 24 : 3684 - 3690
  • [3] Connexin32 as a tumor suppressor gene in a metastatic renal cell carcinoma cell line
    Fujimoto, E
    Sato, H
    Shirai, S
    Nagashima, Y
    Fukumoto, K
    Hagiwara, H
    Negishi, E
    Ueno, K
    Omori, Y
    Yamasaki, H
    Hagiwara, K
    Yano, T
    ONCOGENE, 2005, 24 (22) : 3684 - 3690
  • [4] DIFFERENTIAL PROTEIN-DNA INTERACTIONS ASSOCIATED WITH CELL SPECIFIC EXPRESSION OF RAT CONNEXIN32 (CX32) GENE
    BAI, S
    PIETRANGELO, A
    SPRAY, DC
    BURK, RD
    MOLECULAR BIOLOGY OF THE CELL, 1992, 3 : A293 - A293
  • [5] Liver cell-specific transcriptional regulation of connexin32
    Piechocki, MP
    Toti, RM
    Fernstrom, MJ
    Burk, RD
    Ruch, RJ
    BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 2000, 1491 (1-3): : 107 - 122
  • [6] CELL-PROLIFERATION AND ADVANCEMENT OF HEPATOCARCINOGENESIS IN THE RAT ARE ASSOCIATED WITH A DECREASE IN CONNEXIN-32 EXPRESSION
    TSUDA, H
    ASAMOTO, M
    BABA, H
    IWAHORI, Y
    MATSUMOTO, K
    IWASE, T
    NISHIDA, Y
    NAGAO, S
    HAKOI, K
    YAMAGUCHI, S
    OZAKI, K
    YAMASAKI, H
    CARCINOGENESIS, 1995, 16 (01) : 101 - 105
  • [7] A novel function of connexin 32: marked enhancement of liver function in a hepatoma cell line
    Yang, J
    Ichikawa, A
    Tsuchiya, T
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 307 (01) : 80 - 85
  • [8] Connexin32 gene expression in rat sciatic nerves and cultured Schwann cells
    Satake, M
    Yoshimura, T
    Ohnishi, A
    Kobayashi, T
    DEVELOPMENTAL NEUROSCIENCE, 1997, 19 (02) : 189 - 195
  • [9] GATING PROPERTIES OF CONNEXIN32 CELL CELL CHANNELS AND THEIR MUTANTS EXPRESSED IN XENOPUS OOCYTES
    WERNER, R
    LEVINE, E
    RABADANDIEHL, C
    DAHL, G
    PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1991, 243 (1306) : 5 - 11
  • [10] Growth-suppressive function of human connexin32 in a conditional immortalized mouse hepatocyte cell line
    Kojima, T
    Srinivas, M
    Fort, A
    Urban, M
    Lee, GH
    Sawada, N
    Spray, DC
    IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 2001, 37 (09) : 589 - 598