Development of nine new microsatellite loci for the American beaver, Castor canadensis (Rodentia: Castoridae), and cross-species amplification in the European beaver, Castor fiber

被引:13
|
作者
Pelz-Serrano, Karla [1 ]
Munguia-Vega, Adrian [1 ]
Piaggio, Antoinette J. [4 ]
Neubaum, Melissa [4 ]
Munclinger, Pavel [2 ]
Partl, Adam [2 ]
Van Riper, Charles, III [3 ]
Culver, Melanie [1 ]
机构
[1] Univ Arizona, Sch Nat Resources, Tucson, AZ 85721 USA
[2] Charles Univ Prague, Fac Sci, Dept Zool, CR-12844 Prague 2, Czech Republic
[3] Univ Arizona, USGS SBSC Sonoran Desert Res Stn, Tucson, AZ 85721 USA
[4] Wildlife Serv, Wildlife Genet Lab, Natl Wildlife Res Ctr, USDA, Ft Collins, CO 80521 USA
关键词
beaver; Castor canadensis; Castor fibre; cross-species amplification success; microsatellite; STREAMS; TESTS; PONDS;
D O I
10.1111/j.1755-0998.2008.02364.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We developed nine new nuclear dinucleotide microsatellite loci for Castor canadensis. All loci were polymorphic, except for one. The number of alleles ranged from two to four and from five to 12 in populations from Arizona and Wisconsin, respectively. Average heterozygosity ranged from 0.13 to 0.86 per locus. Since cross-species amplification in Castor fiber was successful only in four loci, we tested also nine recently published C. canadensis loci in the Eurasian species. Eight of the published loci amplified; however, three were monomorphic. The number of alleles was lower in C. fiber than in C. canadensis at all loci tested.
引用
收藏
页码:551 / 554
页数:4
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