Instability of CHO chromosomes containing interstitial telomeric sequences originating from Chinese hamster chromosome 10

被引：32
|
作者：
Slijepcevic, P [1 ]
Xiao, Y [1 ]
Natarajan, AT [1 ]
Bryant, PE [1 ]
机构：
[1] LEIDEN UNIV,DEPT RADIAT GENET & CHEM MUTAGENESIS,NL-2300 RA LEIDEN,NETHERLANDS
来源：
CYTOGENETICS AND CELL GENETICS | 1997年 / 76卷 / 1-2期
关键词：
D O I：
10.1159/000134516
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
We identified a marker chromosome in the CHO K1 cell line containing amplifications of interstitial telomeric sequences originating from Chinese hamster chromosome 10. Analysis of the progression of this chromosome in two subclones of CHO K1 revealed sensitivity of one amplicon to chromosome breakage, resulting in telomere function at the break site. In addition, two more marker chromosomes, both containing amplifications of interstitial telomeric sequences from chromosome 10, were formed during karyotypic evolution of the CHO K1 subclones. The presence of some of the marker chromosomes was also identified in the radiosensitive xrs 5 cell line derived from CHO K1. These results indicate instability of CHO K1 chromosomes containing interstitial telomeric sequences originating from Chinese hamster chromosome 10.
引用
收藏
页码：58 / 60
页数：3
相关论文
共 22 条
  • [1] Bleomycin induces delayed instability of interstitial telomeric sequences in Chinese hamster ovary cells
    Vidal Bravo, Malena
    Bianchi, Martha S.
    Bolzan, Alejandro D.
    MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2012, 731 (1-2) : 133 - 139
  • [2] Molecular organization of internal telomeric sequences in Chinese hamster chromosomes
    Faravelli, M
    Azzalin, CM
    Bertoni, L
    Chernova, O
    Attolini, C
    Mondello, C
    Giulotto, E
    GENE, 2002, 283 (1-2) : 11 - 16
  • [3] The influence of interstitial telomeric sequences on chromosome instability in human cells
    Desmaze, C
    Alberti, C
    Martins, L
    Pottier, G
    Sprung, CN
    Murnane, JP
    Sabatier, L
    CYTOGENETICS AND CELL GENETICS, 1999, 86 (3-4): : 288 - 295
  • [4] Interstitial telomeric sequences in vertebrate chromosomes: Origin, function, instability and evolution
    Bolzan, Alejandro D.
    MUTATION RESEARCH-REVIEWS IN MUTATION RESEARCH, 2017, 773 : 51 - 65
  • [5] Streptonigrin induces delayed chromosomal instability involving interstitial telomeric sequences in Chinese hamster ovary cells
    Mencucci, Maria V.
    Vidal Bravo, Malena
    Bianchi, Martha S.
    Bolzan, Alejandro D.
    MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2012, 747 (01) : 46 - 52
  • [6] Interstitial telomeric sequences are not preferentially involved in the chromosome damage induced by the methylating compound streptozotocin in chinese hamster cells
    Quiroga, Ivana Y.
    Paviolo, Natalia S.
    Bolzan, Alejandro D.
    ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 2013, 54 (02) : 147 - 152
  • [7] Effect of bleomycin on interstitial telomeric sequences of immortalized Chinese hamster ovary cells
    Sanchez, Julieta
    Bianchi, Martha S.
    Bolzan, Alejandro D.
    MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2009, 669 (1-2) : 139 - 146
  • [8] Relationship between heterochromatic interstitial telomeric sequences and chromosome damage induced by the radiornimetic compound streptonigrin in Chinese hamster ovary cells
    Sanchez, Julieta
    Bianchi, Martha S.
    Bolzan, Alejandro D.
    MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2010, 684 (1-2) : 90 - 97
  • [9] ABSENCE OF TERMINAL TELOMERIC FISH SIGNALS IN CHROMOSOMES FROM IMMORTAL CHINESE-HAMSTER CELLS
    SLIJEPCEVIC, P
    BRYANT, PE
    CYTOGENETICS AND CELL GENETICS, 1995, 69 (1-2): : 87 - 89
  • [10] ANALYSIS OF RESTRICTION ENZYME-INDUCED CHROMOSOME-ABERRATIONS IN THE INTERSTITIAL TELOMERIC REPEAT SEQUENCES OF CHO AND CHE CELLS BY FISH
    BALAJEE, AS
    OH, HJ
    NATARAJAN, AT
    MUTATION RESEARCH, 1994, 307 (01): : 307 - 313
123