Lipase-catalyzed synthesis of monoacylglycerides by glycerolysis of campher tree seed oil and cocoa-butter

The ligase-catalyzed glycerolysis of Campher tree seed oil and Cocoa-butter in a solid-phase system was studied. Lipases from Pseudomonas cepacia (PCL) and Chromobacterium viscosum (CVL) were considered as suitable for the synthesis of monoacylglycerols (MAG). The glycerolysis of Campher tree seed oil was performed initially at 25 degrees C followed by cooling to 7 degrees C. This resulted in 86% (PCL) and 90% (CVL) MAG. Maximum concentration of MAG from Cocoa-butter was 89% with both Lipases, when the reaction was performed at 25 degrees C. Isolation of MAG was performed by extraction with chloroform at 4 degrees C followed by purification via silica gel chromatography.