Biosensor Based on Ion Selective Electrode for Detection of L-Arginine in Fruit Juices

被引：15

作者：

Verma, Neelam ^{[1
]}

Singh, Ashish Kumar ^{[1
]}

Kaur, Pawanjit ^{[1
]}

机构：

[1] Punjabi Univ Patiala, Dept Biotechnol, Biosensor Technol Lab, Patiala 147002, Punjab, India

来源：

JOURNAL OF ANALYTICAL CHEMISTRY | 2015年 / 70卷 / 09期

关键词：

microbial arginine deiminase; L-arginine; biosensor; ion selective electrode; fruit juices; CRYSTALLIZATION; OPTIMIZATION; CITRULLINE; DEIMINASE;

D O I：

10.1134/S1061934815090129

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A biosensor has been developed for detection of L-arginine. The biosensor has been constructed using enzyme arginine deiminase as bio-component extracted from Pseudomonas putida MTCC 1313. The immobilization of enzyme was carried out in acrylamide gel, which was fixed onto the ion selective electrode. The optimization and kinetic characterization of free and immobilized enzyme was also studied. The response time of developed biosensor was found < 30 s and a good linear range for arginine concentration from 1 to 10(-9) M was shown. The developed biosensor was successfully applied for detection of arginine content in fruit juices.

引用

页码：1111 / 1115

页数：5

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