Expression of envelope protein (E2) of bovine viral diarrhea virus in insect cells

被引:9
|
作者
Kweon, CH [1 ]
Yoon, YD [1 ]
An, SH [1 ]
Lee, YS [1 ]
机构
[1] SEOUL NATL UNIV,SEOUL 151,SOUTH KOREA
来源
JOURNAL OF VETERINARY MEDICAL SCIENCE | 1997年 / 59卷 / 05期
关键词
BVDV; envelope protein (E2); expression;
D O I
10.1292/jvms.59.415
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The gene encoding the envelope glycoprotein (E2) of bovine viral diarrhea virus (BVDV) was expressed in a baculovirus. The expressed protein was detected on the surface of infected cells by immunofluorescence. Western blotting analysis showed the presence of the expressed protein of a similar molecular size to the E2 protein. The antigenicity of expressed protein were tested in guinea pigs and cattle. The immunized animals developed neutralizing antibodies against BVDV.
引用
收藏
页码:415 / 419
页数:5
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