Effects of supercritical fluid chromatography conditions on enantioselectivity and performance of polyproline-derived chiral stationary phases

被引：12
|
作者：
Novell, Arnau [1 ]
Mendez, Alberto [2 ]
Minguillon, Cristina [1 ]
机构：
[1] Univ Barcelona, E-08921 Santa Coloma De Gramenet, Spain
[2] Waters Cromatog SA, Cerdanyola Del Valles 08290, Spain
来源：
JOURNAL OF CHROMATOGRAPHY A | 2015年 / 1403卷
关键词：
poly-L-proline oligomers; Monolithic columns; Enantioseparation; Chiral stationary phases; Supercritical fluid chromatography; MONOLITHIC SILICA COLUMNS; POLY-L-PROLINE; LIQUID-CHROMATOGRAPHY; MOLECULAR-DYNAMICS; SELECTORS; ENANTIOSEPARATION; OCTAPROLINE; IMPROVEMENT; RESOLUTION;
D O I：
10.1016/j.chroma.2015.05.026
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The chromatographic behaviour and performance of four polyproline-derived chiral stationary phases (CSPs) were tested using supercritical fluid chromatography (SFC). A series of structurally related racemic compounds, whose enantioseparation was proved to be sensitive to the type of mobile phase used in NP-HPLC, were chosen to be tested in the SFC conditions. Good enantioselection ability was shown by the CSPs for the analytes tested in the new conditions. Resolution, efficiency and analysis time, were considerably improved with respect to NP-HPLC when CO2/alcohol mobile phases were used. Monolithic columns clearly show enhanced chromatographic parameters and improved performance respect to their bead-based counterparts. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：138 / 143
页数：6
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