Cryopreservation of Embryonic Axes of Castor Bean Cultivar 'AL-Guarany 2002'

被引：0

作者：

Nogueira, G. F. ^{[1
]}

Paiva, R. ^{[1
]}

Campos, A. C. A. L. ^{[1
]}

Nery, F. C. ^{[1
]}

Vargas, D. P. ^{[1
]}

Paiva, P. D. de O. ^{[1
]}

机构：

[1] Univ Fed Lavras, Dept Biol, Sect Plant Physiol, Lavras, MG, Brazil

来源：

INTERNATIONAL SYMPOSIUM ON CRYOPRESERVATION IN HORTICULTURAL SPECIES | 2011年 / 908卷

关键词：

Ricinus communis; cryoprotectant; micropropagation; PVS2; conservation;

D O I：

暂无

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The castor bean (Ricinus communis) is an oleaginous plant with many applications in the society and economy. Castor bean seeds tolerate storage in seed banks for a short period compared to orthodox seeds, probably due to its high lipid content. This study aimed elaborate a cryopreservation protocol for castor bean embryonic axes. After seeds disinfection, embryonic axes of the cultivar 'AL-Guarany 2002' were isolated, dehydrated and inoculated directly on culture medium (control) or cryostored in liquid nitrogen at -196 degrees C, during one hour, in the presence or absence of cryoprotectant solutions (PVS2 or modified PVS2) prior to inoculation on culture. It was obtained 100% of in vitro regeneration for all treatments. After acclimatization a surviving rate of 100% was obtained for embryonic axes cryostored only in liquid nitrogen. Axes stored in the presence of PVS2 presented a survival rate of 35%. The results obtained showed that the cryopreservation of castor bean embryonic axes in LN without cryoprotectants is a suitable technique to preserve the genetics resources of this species.

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页码：233 / 237

页数：5