Immunochromatographic lateral flow strip for on-site detection of bisphenol A

被引：69
|
作者：
Mei, Zhanlong [1 ]
Deng, Yi [1 ]
Chu, Huaqin [2 ]
Xue, Feng [1 ,3 ]
Zhong, Youhao [1 ]
Wu, Jingjing [1 ]
Yang, Huan [1 ]
Wang, Zhichao [1 ]
Zheng, Lei [1 ]
Chen, Wei [1 ]
机构：
[1] Hefei Univ Technol, Sch Biotechnol & Food Engn, Hefei 230009, Peoples R China
[2] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 210004, Peoples R China
[3] Jiangsu Entry Exit Inspect & Quarantine Bur, Nanjing 200002, Jiangsu, Peoples R China
来源：
MICROCHIMICA ACTA | 2013年 / 180卷 / 3-4期
关键词：
Lateral flow strip; Bisphenol A; On-site detection; Food safety; VISUAL DETECTION; ASSAY; BIOSENSOR; RESIDUES; EXPOSURE; SAMPLES; VIRUS; PAPER;
D O I：
10.1007/s00604-012-0930-2
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We have developed a lateral flow assay (LFA) for the detection of bisphenol A (BPA) in water samples. Antibody against BPA was labeled with gold nanoparticles, and these conjugates were used as the recognition probes for the construction of an LFA strip. The diameter of the gold nanoparticles, the amount of antibody, the pH of the buffer, and the categories of the conjugation pad were optimized. The resulting method has a (visual) detection limit of 5 ppb, and of 0.92 ppb if used in combination with professional software. This LFA displays excellent specificity and was applied to spiked water samples with satisfactory results.
引用
收藏
页码：279 / 285
页数：7
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