Manipulation of internodal segments of F1 tomato hybrids raised in vitro under different regimes of plant growth regulators

The relative morphogenetic response of internodal segments of tomato hybrids viz., Bornia and Royesta was studied at varying concentration of BAP, Kn, NAA and IAA. NAA @ 3 mu M/l proved to be better than IAA @ 3 mu M/l in combination with different PGR regimes of both BAP (0, 3, 6, 9 mu M/l) as well as Kn (0, 6, 12, 18 mu M/l) exhibiting maximum callus formation in bath the hybrids whereas its response for root regeneration was not satisfactory. The influence of IAA in combination with both cytokinins was comparatively less callogenetic but for root regeneration its response was better than NAA. A little shoot regeneration in both hybrids was observed when callus was subjected to regeneration medium containing 2 mg/l and 0.1 mg/l of IBA.