Fabrication of Photo-polymerized PCL Tissue Engineering Scaffolds by Dynamic Masking Rapid Prototyping System

Polycaprolactone (PCL) is one of the popular biomaterials used in tissue engineering scaffolds, but it is seldom shaped by photo-polymerization. Layered manufacturing techniques, also known as Rapid Prototyping (RP) processes, provide a great opportunity to fabricate 3D scaffolds without problems such as limited control of pore-size and restricted geometric shapes in traditional methods. In our previous researches, the Biomedical Dynamic Masking Rapid Prototyping System was developed to photo-cure biodegradable materials through visible light. In this research, the Dynamic Masking RP System was modified to photo-polymerize cross-linkable PCL to form tissue engineering scaffolds. The cross-linkable PCL was synthesized by reacting PCL and acryloyl chloride, and dissolved in acetone mixing with photo-initiator. The tensile test and degradation test were performed on the cured PCL samples. Fabrication of single-layer pattern was first tested to understand the system's capability and showed the errors were within 7%. Two types of scaffold design concepts were adopted-one took square, hexagon, or triangle as a basic element to create 2D grid patterns, and the interconnected pore were produced by offsetting the 2D pattern in alternating layers; the other took a double-sided trapezoid as a unit and arrayed it into tube shape with interconnected pore network. Various PCL porous tube scaffolds have been successfully fabricated in this study. In the future, they can be utilized to cell growth or mass cell duplication applications.