Nocardia mangyaensis sp. nov., a novel actinomycete isolated from crude-oil-contaminated soil

A Gram-stain-positive, aerobic, non-motile and mycolic-acid-containing strain, designated Y48(T), was isolated from soil contaminated by crude oil located in the northern margin of the Qaidam Basin. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Y48(T) belongs to the genus Nocardia and is closely related to N. cummidelens DSM 44490(T) (99.0 % similarity), N. soli DSM 44488(T) (99.0 %), N. lasii 3C-HV12(T) (98.9 %), N. salmonicida NBRC 13393(T) (98.6 %), N. ignorata NBRC 108230(T) (98.6 %) and N. coubleae NBRC 108252(T) (98.6 %). The average nucleotide identity and DNA-DNA hybridization values between strain Y48(T) and the reference strains were 75.9-84.5 and 27.5-29.0 %, respectively, values that were below the thresholds for species delineation. Chemotaxonomic analysis indicated that the major fatty acids of strain Y48(T) were C-16(:0), summed feature 3 (C-16:1 omega 6c/C-16:1 omega 7c), C-18:1 omega 9c and C-18:0 10 methyl (TBSA). The respiratory quinone was MK-8(H-4,H-omega-cycl). The polar lipid profile was composed of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, two glycolipids and three unidentified lipids. The cell-wall hydrolysates contained mesodiaminopimelic acid, with ribose, arabinose, glucose and galactose as whole-cell sugars. A combination of 16S rRNA gene sequence analysis, and phenotypic and chemotaxonomic characterizations demonstrated that strain Y48(T) represents a novel species of the genus Nocardia, for which the name Nocardia mangyaensis sp. nov. is proposed. The type strain is Y48(T) (=JCM 32795(T)=CGMCC 4.7494(T)).