Reversible spatial and temporal control of lipid signaling

被引：6

作者：

Gaur, Pankaj ^{[1
]}

Galkin, Maksym ^{[1
]}

Hauke, Sebastian ^{[2
]}

Redkin, Ruslan ^{[1
]}

Barnes, Carolyn ^{[1
]}

Shvadchak, Volodymyr V. ^{[1
]}

Yushchenko, Dmytro A. ^{[1
,3
]}

机构：

[1] Czech Acad Sci, Lab Chem Biol, Inst Organ Chem & Biochem, Flemingovo Namesti 2, Prague 16610 6, Czech Republic

[2] European Mol Biol Lab EMBL, Cell Biol & Biophys Unit, Meyerhofstr 1, D-69117 Heidelberg, Germany

[3] Miltenyi Biotech GmbH, Grp Bioconjugat Chem, Friedrich Ebert Str 68, D-51429 Bergisch Gladbach, Germany

来源：

CHEMICAL COMMUNICATIONS | 2020年 / 56卷 / 73期

关键词：

OPTICAL CONTROL; PROTEIN; PHOTORELEASE;

D O I：

10.1039/d0cc04146g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Herein, we introduce versatile molecular tools that enable specific delivery and visualization of photoswitchable lipids at cellular membranes, namely at the plasma membrane and internal membranes. These molecules were prepared by tethering ortho-nitrobenzyl-based fluorescent cages with a signaling lipid bearing an azobenzene photo-switch. They permit two sequential photocontrolled reactions, which are uncaging of a lipid analogue and then its repeated activation and deactivation. We used these molecules to activate GPR40 receptor transiently expressed in HeLa cells and demonstrated downstream modulation of intracellular Ca2+ levels.

引用

页码：10646 / 10649

页数：4

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