The structure of the third intracellular loop of the muscarinic acetylcholine receptor M2 subtype

被引：21

作者：

Ichlyama, S

Oka, Y

Haga, K

Kojima, S

Tateishi, Y

Shirakawa, M

Haga, T

机构：

[1] Gakushuin Univ, Inst Biomol Sci, Toshima Ku, Tokyo 1718588, Japan

[2] Kyoto Univ, Dept Mol Engn, Nishigyo Ku, Kyoto 6158510, Japan

来源：

FEBS LETTERS | 2006年 / 580卷 / 01期

关键词：

circular dichroism; G protein-coupled receptor; secondary structure;

D O I：

10.1016/j.febslet.2005.11.042

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have examined whether the long third intracellular loop (i3) of the muscarinic acetylcholine receptor M-2 subtype has a rigid structure. Circular dichroism (CD) and nuclear magnetic resonance spectra of M(2)i3 expressed in and purified from Escherichia coli indicated that M(2)i3 consists mostly of random coil. In addition, the differential CD spectrum between the M-2 and M-2 Delta i3 receptors, the latter of which lacks most of i3 except N- and C-terminal ends, gave no indication of secondary structure. These results suggest that the central part of i3 of the M-2 receptor has a flexible structure. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：23 / 26

页数：4

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