Spectrophotometric determination of zinc with phenylfluorone in the presence of triton X-100 and cetylpyridinium chloride

被引:0
|
作者
Winkler, W [1 ]
Arenhövel-Pacula, A [1 ]
机构
[1] Silesian Univ, Inst Chem, PL-40006 Katowice, Poland
来源
CHEMIA ANALITYCZNA | 1999年 / 44卷 / 04期
关键词
zinc; phenylfluorone; Triton X-100; cetylpyridinium chloride; insulin; spectrophotometry;
D O I
暂无
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Spectrophotometric determination of zinc using the reaction with phenylfluorone (PF) in the presence of cetylpyridinium chloride (CP) and Triton X-100 was elaborated. Optimum concentrations of PF, CP and Triton X-100, and pH ensuring maximum absorbance were defined. The effect of foreign ions was elucidated. The maximum absorbance is observed at 573 nm with the molar absorptivity 1.09x10(5) l mol(-1) cm(-1). The Beer's law is obeyed for zinc concentrations in the range 0.04-0.4 mu g ml(-1). The method has been applied for determination of zinc in insulin.
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页码:725 / 730
页数:6
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