The potential application and challenge of powerful CRISPR/Cas9 system in cardiovascular research

CRISPR/Cas9 is a precision-guided munition found in bacteria to fight against invading viruses. This technology has enormous potential applications, including altering genes in both somatic and germ cells, as well as generating knockout animals. Compared to other gene editing techniques such as zinc finger nucleases and TALENS, CR1SPR/Cas9 is much easier to use and highly efficient. Importantly, the multiplex capacity of this technology allows multiple genes to be edited simultaneously. CRISPR/Cas9 also has the potential to prevent and cure human diseases. In this review, we wish to highlight some key points regarding the future prospect of using CRISPRICas9 as a powerful tool for cardiovascular research, and as a novel therapeutic strategy to treat cardiovascular diseases. (C) 2016 Elsevier Ireland Ltd. All rights reserved.