OPTIMIZATION OF CONDITIONS FOR ENZYMATIC PRODUCTION OF COLLAGEN HYDROLYSATES FROM A LOW-VALUE ACAUDINA MOLPADIOIDES AND THEIR ACTIVITIES

Statistics-based optimization of conditions for enzymatic production of collagen hydrolysates from a low-value Acaudina molpadioides was carried out, and their activities were also evaluated. The highest hydrolysis degree of collagen was 17.01% when substrate concentration, temperature and pH were 34.3g/g (dry sea cucumber powder weight/enzyme weight), 43.2C and 7.12, respectively. A second-order polynomial model was established to depict the relationship of the hydrolysis degree of collagen and factors. The IC50 values of OH, O-2(-) and 1,1-diphenyl-2-picrylhydrazyl scavenged by the purified P1 component from collagen hydrolysates were 2.77, 5.08 and 3.59mg/mL, respectively. Collagen hydrolysates could obviously increase the survival rate of injured SK-N-SH cells by 11.4% at 500g/mL. Practical ApplicationsCollagen hydrolysates can be effectively prepared from A.molpadioides using papain under the optimal conditions obtained by the response surface methodology and are demonstrated to be extremely potential candidates for novel antioxidants and drugs for preventing the injury of nerve cells induced by hydrogen peroxide.