Stromal expression of hemopexin is associated with lymph-node metastasis in pancreatic ductal adenocarcinoma

被引:12
|
作者
Suzuki, Yukie [1 ]
Takadate, Tatsuyuki [1 ]
Mizuma, Masamichi [1 ]
Shima, Hiroki [2 ]
Suzuki, Takashi [3 ]
Tachibana, Tomoyoshi [1 ]
Shimura, Mitsuhiro [1 ]
Hata, Tatsuo [1 ]
Iseki, Masahiro [1 ]
Kawaguchi, Kei [1 ]
Aoki, Takeshi [1 ]
Hayashi, Hiroki [1 ]
Morikawa, Takanori [1 ]
Nakagawa, Kei [1 ]
Motoi, Fuyuhiko [1 ]
Naitoh, Takeshi [1 ]
Igarashi, Kazuhiko [2 ]
Unno, Michiaki [1 ]
机构
[1] Tohoku Univ, Dept Surg, Grad Sch Med, Sendai, Miyagi, Japan
[2] Tohoku Univ, Dept Biochem, Grad Sch Med, Sendai, Miyagi, Japan
[3] Tohoku Univ, Dept Pathol & Histotechnol, Grad Sch Med, Sendai, Miyagi, Japan
来源
PLOS ONE | 2020年 / 15卷 / 07期
基金
日本学术振兴会;
关键词
PROTEOMIC ANALYSIS; CURATIVE RESECTION; CANCER-PATIENTS; DISEASE; FIBROBLASTS; PROTEINS; NOMOGRAM; MARKERS; RATIO;
D O I
10.1371/journal.pone.0235904
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Pancreatic ductal adenocarcinoma is one of the most aggressive types of cancer. Certain proteins in the tumor microenvironment have attracted considerable attention owing to their association with tumor invasion and metastasis. Here, we used proteomics to identify proteins associated with lymph-node metastasis, which is one of the prognostic factors. We selected lymph node metastasis-positive and -negative patients (n = 5 each) who underwent pancreatectomy between 2005 and 2015 and subjected to comprehensive proteomic profiling of tumor stroma. A total of 490 proteins were detected by mass spectrometry. Software analysis revealed that nine of these proteins were differentially expressed between the two patient groups. We focused on hemopexin and ferritin light chain based on immunohistochemistry results. We assessed the clinicopathological data of 163 patients and found that hemopexin expression was associated with UICC N2 (p= 0.0399), lymph node ratio (p= 0.0252), venous invasion (p= 0.0096), and lymphatic invasion (p= 0.0232). Notably,in vitroassays showed that hemopexin promotes invasion of the pancreatic cancer cells. Our findings suggest that hemopexin is a lymph node metastasis-associated protein that could potentially serve as a useful therapeutic target or biomarker of pancreatic ductal adenocarcinoma.
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页数:17
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