Anthrax toxin receptor 2a controls mitotic spindle positioning

被引：39
|
作者：
Castanon, I. [2 ,3 ]
Abrami, L. [1 ]
Holtzer, L. [2 ,3 ]
Heisenberg, C. P. [4 ]
van der Goot, F. G. [1 ]
Gonzalez-Gaitan, M. [2 ,3 ]
机构：
[1] Ecole Polytech Fed Lausanne, Global Hlth Inst, CH-1015 Lausanne, Switzerland
[2] Dept Biochem, CH-1211 Geneva 4, Switzerland
[3] Dept Mol Biol, CH-1211 Geneva 4, Switzerland
[4] IST Austria, A-3400 Klosterneuburg, Austria
来源：
NATURE CELL BIOLOGY | 2013年 / 15卷 / 01期
关键词：
CELL-DIVISION ORIENTATION; ZEBRAFISH GASTRULATION; RHO GTPASES; F-ACTIN; EXTENSION MOVEMENTS; IN-VIVO; POLARITY; PROTEIN; EXPRESSION; MECHANISM;
D O I：
10.1038/ncb2632
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Oriented mitosis is essential during tissue morphogenesis. The Wnt/planar cell polarity (Wnt/PCP) pathway orients mitosis in a number of developmental systems, including dorsal epiblast cell divisions along the animal-vegetal (A-V) axis during zebrafish gastrulation. How Wnt signalling orients the mitotic plane is, however, unknown. Here we show that, in dorsal epiblast cells, anthrax toxin receptor 2a (Antxr2a) accumulates in a polarized cortical cap, which is aligned with the embryonic A-V axis and forecasts the division plane. Filamentous actin (F-actin) also forms an A-V polarized cap, which depends on Wnt/PCP and its effectors RhoA and Rock2. Antxr2a is recruited to the cap by interacting with actin. Antxr2a also interacts with RhoA and together they activate the diaphanous-related formin zDia2. Mechanistically, Antxr2a functions as a Wnt-dependent polarized determinant, which, through the action of RhoA and zDia2, exerts torque on the spindle to align it with the A-V axis.
引用
收藏
页码：28 / U71
页数：23
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