Genetic transformation of sweet potato (Ipomoea batatas (L.) lam.) by Agrobacterium tumefaciens

被引：4
|
作者：
Otani, M [1 ]
Wakita, Y [1 ]
Shimada, T [1 ]
机构：
[1] Ishikawa Agr Coll, Res Inst Agr Resources, Nonoichi, Ishikawa 9218836, Japan
来源：
PROCEEDINGS OF THE 4TH INTERNATIONAL SYMPOSIUM ON IN VITRO CULTURE AND HORTICULTURAL BREEDING | 2001年 / 560期
关键词：
transformation; sweet potato; Agrobacterium tumefaciens; transgenic plants;
D O I：
10.17660/ActaHortic.2001.560.35
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Transgenic sweet potato plants were obtained from embryogenic calli following Agrobacterium tumefaciens-mediated transformation. A. tumefaciens strain EHA101/pIG121-Hm used in the present study contained a binary vector with genes for gusA and hygromycin resistance (hpt). Around ten hygromycin resistant cell clusters were produced from 1 g fresh weight of the infected embryogenic calli. The hygromycin resistant plantlets were regenerated from 53.1% of the hygromycin-resistant calli. Histochemical GUS assay and Southern hybridization analysis indicated that these plants were stably transformed with a copy number of introduced genes of one to three. Transgenic plants grew normally and formed storage roots after 3 months of cultivation in a green house. We have introduced some agronomically important genes by using our transformation system.
引用
收藏
页码：193 / 196
页数：4
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