Long non-coding RNA OIP5-AS1 plays an oncogenic role in ovarian cancer through targeting miR-324-3p/NFIB axis

被引：22
|
作者：
Liu, Q-Y [1 ]
Jiang, X-X [2 ]
Tian, H-N [3 ]
Guo, H-L [1 ]
Guo, H. [4 ]
Guo, Y. [1 ]
机构：
[1] Rizhao Peoples Hosp, Dept Gynaecol, Rizhao, Peoples R China
[2] Yantaishan Hosp, Dept Oncol 2, Yantai, Peoples R China
[3] Huantai Cty Peoples Hosp, Phys Examinat Ctr, Zibo, Peoples R China
[4] Peoples Hosp Zhangqiu Area, Hlth Management Ctr, Jinan, Peoples R China
来源：
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES | 2020年 / 24卷 / 13期
关键词：
Ovarian cancer; OIP5AS1; MiR-324-3p; NFIB; NUCLEAR FACTOR I/B; EXPRESSION; PROLIFERATION; PROGRESSION; CELLS; NFIB;
D O I：
10.26355/eurrev_202007_21881
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
OBJECTIVE: Long non-coding RNAs (lncRNAs) have been found to exert specific functions in the progression of ovarian cancer (OC), except for lncRNA-OIP5-AS1. In this study, we aim at exploring the molecular mechanisms of OIP5-AS1 in OC. PATIENTS AND METHODS: The expression levels of OIP5-AS1, miR-324-3p, and NFIB in OC tissues and OC cell lines were explored by qRT-PCR assay. The OC cell vitality was examined by CCK-8 and transwell assay. The protein expression level of NFIB was measured by Western blot analysis. The correlation between OIP5-AS1, miR-324-3p, and NFIB was appraised by Dual-Luciferase reporter assay. RESULTS: OIP5-AS1 and NFIB were validated to be upregulated in both OC tissues and OC cell lines. Inversely, miR-324-3p downregulation was found in OC tissues and OC cell lines. Functionally, OIP5-AS1 knockdown and miR-324-3p overexpression restrained SKOV3 cell viability, invasion, and migration. Our results verified that OIP5-AS1 inhibited the expression of miR-3243p in OC. Moreover, miR-324-3p directly targets NFIB. Besides that, NFIB silencing restrained the progression of SKOV3 cells. CONCLUSIONS: The present study clarified that OIP5-AS1 accelerated OC progression by sponging miR-324-3p and upregulating NFIB. OIP5-AS1 can be a possible therapeutic target for the treatment of OC.
引用
收藏
页码：7266 / 7275
页数：10
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