Expression of the alpha 7 subunit of the nicotinic acetylcholine receptor in normal and myasthenic human thymuses

被引:0
|
作者
Navaneetham, D
Penn, A
Howard, J
ContiFine, BM
机构
[1] UNIV MINNESOTA,DEPT BIOCHEM,ST PAUL,MN 55108
[2] UNIV MINNESOTA,DEPT PHARMACOL,MINNEAPOLIS,MN 55455
[3] NINCDS,NIH,OFF DIRECTOR,BETHESDA,MD 20892
[4] UNIV N CAROLINA,DEPT NEUROL,CHAPEL HILL,NC 27599
关键词
acetylcholine receptor; alpha; 7; subunit; human thymus; myasthenia gravis;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The nicotinic acetylcholine receptor (AChR) is a transmembrane glycoprotein composed of five homologous subunits. Different isoforms of the AChR alpha subunit exist (alpha 1 to alpha 9). Of them, alpha 1 is expressed in muscle, alpha 2 to alpha 9 in neuronal cells. Muscle AChR is the target autoantigen in the autoimmune disease myasthenia gravis (MG). The thymus is implicated in MG pathogenesis, and the anti-AChR autoimmune response may start in this tissue, that expresses the muscle-type alpha 1 subunit as well as other muscle AChR subunits. The thymus also expresses the ''neuronal'' alpha 3 and alpha 5 subunits. By using polymerase chain reaction and other molecular techniques, we demonstrate here expression of the AChR alpha 7 subunit transcript in thymuses from both myasthenic patients and normal subjects. The alpha 7 subunit can form homo-oligomeric functional AChR complexes that, like muscle AChR, bind alpha-bungarotoxin. The demonstration of expression of the alpha 7 subunit in the thymus suggests that alpha-bungarotoxin binding, functional AChRs of the neuronal type are normally present in the thymus.
引用
收藏
页码:433 / 442
页数:10
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