Specific detection of all members of the Venezuelan Equine Encephalitis complex: Development of a RT-Nested PCR

被引：4

作者：

Belen Pisano, Maria ^{[1
]}

Sanchez Seco, Maria Paz ^{[2
]}

Elizabeth Re, Viviana ^{[1
]}

Farias, Adrian A. ^{[1
]}

Silvia Contigiani, Marta ^{[1
]}

Tenorio, Antonio ^{[2
]}

机构：

[1] Univ Nacl Cordoba, Fac Ciencias Med, Inst Virol Dr JM Vanella, RA-5016 Cordoba, Argentina

[2] Inst Salud Carlos III, Ctr Nacl Microbiol, Madrid, Spain

来源：

JOURNAL OF VIROLOGICAL METHODS | 2012年 / 186卷 / 1-2期

关键词：

VEEV; Alphavirus; RT-Nested PCR; Arbovirus; Diagnostic; Surveillance; PIXUNA;

D O I：

10.1016/j.jviromet.2012.05.009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Venezuelan Equine Encephalitis (VEE) complex belongs to alphavirus genus in the family Togaviridae. Several species of this complex are pathogenic to humans. VEE infections can produce severe or mild disease, and many cases remain undiagnosed. A specific and sensitive reverse transcriptase nested polymerase chain reaction (RT-Nested PCR) method was developed for the detection of all VEE subtypes, including Rio Negro Virus (RNV) (subtype VI), which circulates only in Argentina. Degenerated primers were designed and thermal cycling parameters were standardized. This technique is suitable for rapid and specific detection of these viruses, and may be useful for diagnosis and surveillance. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：203 / 206

页数：4

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