Antimicrobial and antioxidant activity of proteins fromFeijoa sellowianaBerg. fruit before and after in vitro gastrointestinal digestion

被引:25
|
作者
Piscopo, Marina [1 ]
Tenore, Gian Carlo [2 ]
Notariale, Rosaria [1 ]
Maresca, Viviana [1 ]
Maisto, Maria [2 ]
De Ruberto, Francesca [1 ]
Heydari, Milad [3 ]
Sorbo, Sergio [4 ]
Basile, Adriana [1 ]
机构
[1] Univ Naples Federico II, Dept Biol, Naples, Italy
[2] Univ Napoli Federico II, Dept Farmacy, Naples, Italy
[3] Univ Tehran, Coll Agr & Nat Resources, Dept Agron & Plant Breeding, Karaj, Iran
[4] Univ Naples Federico II, Microscopy Sect, CeSMA, Naples, Italy
关键词
antimicrobial activity; antioxidant activity; in vitro gastrointestinal digestion; Feijoa sellowiana; protein fraction; ACETONIC EXTRACT; CHAETOPTERUS-VARIOPEDATUS; RELEVANCE; SPERM;
D O I
10.1080/14786419.2018.1543686
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
In this paper we report for the first time a method for the extraction of the protein fraction fromFeijoa sellowianaBerg. fruit and its electrophoretic analysis. In addition we analyzed the protein fraction for its antioxidant activity and its effectiveness against different Gram-positive and Gram-negative bacteria both as American type culture collection (ATCC) standard and clinically isolated strains. Both antimicrobial and antioxidant activity resulted stronger respect to that previously obtained for the acetonic extract from the same fruit. Further, we study both activities also on the product of in vitro gastrointestinal digestion ofF. sellowianafruit proteins. The results showed that in vitro gastrointestinal digestion increased 10 fold the antioxidant activity, while the antimicrobial activity, tested only on ATCC strains, resulted from 2 to 4 fold increased. MTT assays showed the non-toxicity of these proteins both before and after digestion.
引用
收藏
页码:2607 / 2611
页数:5
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