Ultrahigh-throughput single-molecule spectroscopy and spectrally resolved super-resolution microscopy

被引：7
|
作者：
Zhang, Zhengyang [1 ,2 ]
Kenny, Samuel J. [1 ]
Hauser, Margaret [1 ]
Li, Wan [1 ]
Xu, Ke [1 ,2 ]
机构：
[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Life Sci, Berkeley, CA 94720 USA
来源：
NATURE METHODS | 2015年 / 12卷 / 10期
关键词：
OPTICAL RECONSTRUCTION MICROSCOPY; FLUORESCENCE NANOSCOPY; ROOM-TEMPERATURE; PROBES; FLUOROPHORES; STORM;
D O I：
10.1038/NMETH.3528
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
By developing a wide-field scheme for spectral measurement and implementing photoswitching, we synchronously obtained the fluorescence spectra and positions of 106 single molecules in labeled cells in minutes, which consequently enabled spectrally resolved, 'true-color' super-resolution microscopy. The method, called spectrally resolved stochastic optical reconstruction microscopy (SR-STORM), achieved cross-talk free three-dimensional (3D) imaging for four dyes 10 nm apart in emission spectrum. Excellent resolution was obtained for every channel, and 3D localizations of all molecules were automatically aligned within one imaging path.
引用
收藏
页码：935 / 938
页数：4
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