Semaphorin 6D regulate corralling, hematoma compaction and white matter injury in mice after intracerebral hemorrhage

Objectives: The Semaphorin 6D (SEMA6D) shows important roles in cell guidance and lipid metabolism, but the effects and mechanisms of SEMA6D on tissue repair, white matter injury and the recovery of neurological function after intracerebral hemorrhage have not been well studied. Materials and Methods: In this study, the autologous whole blood injection model of intracerebral hemorrhage was estab-lished in C57 male mice. SEMA6D knockout CRISPR utilized in the study. Assess-ments included neurological score evaluation and immunofluorescence. Results: SEMA6D increased and peaked at 7d after intracerebral hemorrhage, and mainly located in neurons, microglia and astrocytes. SEMA6D knockout CRISPR aggravated neurological function and showed signs of poorer corralling and hema-toma resolution, with more compartments of well-established physical barrier and more extensive GFAP positive astrocytic border. Furthermore, SEMA6D can pre-vent the decrease of NF-H in the peri-hematoma region, while SEMA6D knockout aggravated WMI. Conclusions: Our study suggested that SEMA6D could influence the recovery of neurological function by regulating the corralling, hematoma com-paction and WMI in mice after intracerebral hemorrhage.