Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive

被引：14

作者：

Zielinski, Kara A. ^{[1
,10
]}

Prester, Andreas ^{[2
]}

Andaleeb, Hina ^{[3
,11
]}

Bui, Soi ^{[4
]}

Yefanov, Oleksandr ^{[1
]}

Catapano, Lucrezia ^{[4
,5
]}

Henkel, Alessandra ^{[1
]}

Wiedorn, Max O. ^{[1
]}

Lorbeer, Olga ^{[6
]}

Crosas, Eva ^{[6
]}

Meyer, Jan ^{[6
]}

Mariani, Valerio ^{[1
,12
]}

Domaracky, Martin ^{[1
]}

White, Thomas A. ^{[6
]}

Fleckenstein, Holger ^{[1
]}

Sarrou, Iosifina ^{[1
,13
]}

Werner, Nadine ^{[3
]}

Betzel, Christian ^{[3
,7
]}

Rohde, Holger ^{[2
]}

Aepfelbacher, Martin ^{[2
]}

Chapman, Henry N. ^{[1
,7
,8
]}

Perbandt, Markus ^{[3
]}

Steiner, Roberto A. ^{[4
,9
]}

Oberthuer, Dominik ^{[1
]}

机构：

[1] Deutsch Elekt Synchrotron DESY, Ctr Free Electron Laser Sci CFEL, Notkestr 85, D-22607 Hamburg, Germany

[2] Univ Med Ctr Hamburg Eppendorf, Inst Med Microbiol Virol & Hyg, Martinistr 52, D-20246 Hamburg, Germany

[3] Univ Hamburg, Inst Biochem & Mol Biol, Lab Struct Biol Infect & Inflammat, DESY, Bldg 22a,Notkestr 85, D-22603 Hamburg, Germany

[4] Kings Coll London, Randall Ctr Cell & Mol Biophys, London, England

[5] MRC Lab Mol Biol, Francis Crick Ave, Cambridge CB2 0QH, England

[6] Deutsch Elektronen Synchrotron DESY, Notkestr 85, D-22607 Hamburg, Germany

[7] Univ Hamburg, Hamburg Ctr Ultrafast Imaging, Luruper Chaussee 149, D-22761 Hamburg, Germany

[8] Univ Hamburg, Dept Phys, Luruper Chaussee 149, D-22761 Hamburg, Germany

[9] Univ Padua, Dept Biomed Sci, Via Ugo Bassi 58-B, I-35131 Padua, Italy

[10] Cornell Univ, Sch Appl & Engn Phys, Ithaca, NY 14853 USA

[11] Bahauddin Zakariya Univ, Dept Biochem, Multan 60800, Punjab, Pakistan

[12] SLAC Natl Accelerator Lab, Linac Coherent Light Source, Menlo Pk, CA 94025 USA

[13] Wiley VCH, Berlin, Germany

来源：

IUCRJ | 2022年 / 9卷

基金：

英国生物技术与生命科学研究理事会; 英国科学技术设施理事会;

关键词：

serial crystallography; partial reflections; protein structures; sample delivery; room temperature; CFEL TapeDrive; K. pneumoniae CTX-M-14; N. haematococca GH11 xylanase; A. flavus urate oxidase; LIPIDIC CUBIC PHASE; RADIATION-DAMAGE; MACROMOLECULAR CRYSTALLOGRAPHY; MILLISECOND CRYSTALLOGRAPHY; SAMPLE DELIVERY; REFINEMENT; PROTEINS; CRYSTALS;

D O I：

10.1107/S2052252522010193

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Serial crystallography at conventional synchrotron light sources (SSX) offers the possibility to routinely collect data at room temperature using micrometre-sized crystals of biological macromolecules. However, SSX data collection is not yet as routine and currently takes significantly longer than the standard rotation series cryo-crystallography. Thus, its use for high-throughput approaches, such as fragment-based drug screening, where the possibility to measure at physiological temperatures would be a great benefit, is impaired. On the way to high-throughput SSX using a conveyor belt based sample delivery system - the CFEL TapeDrive - with three different proteins of biological relevance (Klebsiella pneumoniae CTX-M-14 beta-lactamase, Nectria haematococca xylanase GH11 and Aspergillus flavus urate oxidase), it is shown here that complete datasets can be collected in less than a minute and only minimal amounts of sample are required.

引用

页码：778 / 791

页数：14

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